BARORECEPTOR/HORMONAL INTERACTIONS

这项建议的目的是探索压力感受器的性质。 对清醒动物内分泌功能的影响。重点放在 自从有证据表明神经垂体轴之间存在着相互作用 压力感受性神经与中枢肽能系统的关系。 实验的目的是验证压力感受器输入具有 对加压素和催产素分泌的抑制作用 通过脑干去甲肾上腺素能输入。去窦弓神经将被用作 模型，以探讨去掉传入输入对认知功能的影响。 肽能系统。具体目标是：1)确定 压力感受器去神经对老年人心血管和内分泌功能的影响 清醒的老鼠。血浆加压素和血管紧张素转换酶变化的时程 将评估催产素和心血管参数；2)评估 压力感受器失神经患者的激素和心血管反应 动物使用刺激或抑制分泌的范例；3) 检查去神经动物中枢反应性的变化 使用体内微透析系统将刺激传递到 脑室旁区和视上区；4)通过 通过评估压力感受器输入改变多肽分泌的效果 脑干或下丘脑损害和选择性神经失神经 内分泌和心血管反应；以及5)检查 确定下丘脑/脑干相互作用的影响 脑室旁小细胞区的特殊病变 原子核。 这项调查将提供有关两国之间相互作用的信息 自主神经系统和内分泌下丘脑是至关重要的 在心血管液体/电解质平衡中。

The objective of this proposal is to explore the nature of baroreceptor influences on endocrine function in the conscious animal. The focus is on the neurohypophyseal axis since evidence shows that there is a reciprocal relationship between baroreceptor nerves and central peptidergic systems. Experiments are designed to test the hypothesis that baroreceptor input has inhibitory effects on vasopressin and oxytocin secretion which are mediated by brain stem noradrenergic input. Sinoaortic denervation will be used as the model, to probe the effect of removal of afferent input on the peptidergic systems. The specific aims are : 1) to determine the effect of baroreceptor denervation on cardiovascular and endocrine function in the conscious rat. The time course of the changes in plasma vasopressin and oxytocin and cardiovascular parameters will be evaluated; 2) to evaluate hormonal and cardiovascular responsiveness in the baroreceptor denervated animal using paradigms in which secretion is stimulated or inhibited; 3) to examine the changes in central responsiveness in the denervated animal using an in vivo microdialysis system for the delivery of stimuli to the paraventricular and supraoptic regions; 4) to determine the mechanisms by which baroreceptor input alters peptide secretion by evaluating the effects of brain stem or hypothalamic lesions and selective neural denervation on endocrine and cardiovascular responses; and 5) to examine the hypothalamic/brain stem interactions by determining the influence of specific lesions of the parvicellular region of the paraventricular nucleus. This investigation will provide information on the interactions between the autonomic nervous system and the endocrine hypothalamus which are critical in cardiovascular fluid/electrolyte balance.