FATS, MICROCIRCULATION, & PLATELET-ENDOTHELIAL CELLS

这项研究的广泛，长期目标是测试的影响， 不同的脂肪对微血管事件的影响，因为它们与心血管 疾病 更直接地，我们将测试富含特定脂肪酸的脂肪 (FA)异构体，在存在或不存在抗氧化剂维生素E时， 1）毛细血管血细胞速度（CBV），2）血流量和3） 血小板-内皮和脂蛋白-内皮细胞相互作用。 具体目标是检验以下假设：1）等热量 富含多不饱和脂肪酸（n-3，n-6家族）的脂肪量增加 CBV，皮下和总手指血流量在人类超过饱和 或单不饱和FA，2）在人类中CBV逐渐增加 通过不同水平的n-3和n-6与n-9 FA或西方饮食脂肪 混合物（WDFB-牛肉、猪肉和植物脂肪的组合），3）在 人类添加维生素E的鱼油补充剂与天然 鱼油会增加CBV，减少血小板聚集， 抗脂蛋白诱导的内皮细胞损伤（WDFB将被使用 作为对照），以及4）鱼油（n-3丰富）与橄榄油（n-9 富含）、红花油（富含n-6）或WDFB喂养小鼠， 体内血小板-内皮细胞粘附和血小板聚集。 [This将在有和没有补充维生素E的情况下进行测试， 抗氧化剂对高度可氧化的饮食脂质（鱼， 红花）与更稳定的脂质来源（橄榄，WDFB）。 测试这些 假设人类受试者和小鼠将被给予富含 n-6、n-3、n-9和饱和脂肪酸以及在不同温度下的测量 与上述心血管疾病相关的时间间隔 参数 所获得的知识将使我们能够更好地理解 导致动脉粥样硬化发展的事件， 血栓溶解性疾病

The broad, long-term objectives of this study are to test the effects of different fats on microvascular events as they relate to cardiovascular disease. More directly, we will test fats, rich in specific fatty acid (FA) isomers, in the presence or absence of the antioxidant vitamin E, on 1) capillary blood cell velocity (CBV), 2) blood flow and 3) platelet-endothelial and lipoprotein-endothelial cell interactions. Specific aims are to test the following hypotheses: 1) that isocaloric amounts of fats rich in polyunsaturated FA (n-3, n-6 families) increase CBV, subdermal and total finger blood flow in humans more than saturated or monounsaturated FA, 2) that in humans CBV is incrementally increased by different levels of n-3 and n-6 vs. n-9 FA or a western diet fat blend (WDFB-combination of beef, pork and vegetable fats), 3) that in humans the addition of vitamin E to a fish oil supplement vs. native fish oil will increase CBV, decrease platelet aggregation and protect against lipoprotein induced endothelial cell injury (WDFB will be used as the control), and 4) that fish oil (n-3 rich) vs. olive oil (n-9 rich), safflower oil (n-6 rich) or WDFB fed to mice will decrease in vivo platelet-endothelial cell adherence and platelet aggregation. [This will be tested with and without supplemental vitamin E to test the effects of an antioxidant on highly oxidizable dietary lipids (fish, safflower) vs. a more stable lipid source (olive, WDFB).] To test these hypotheses human subjects and mice will be given fat supplements rich in n-6, n-3, n-9 and saturated fatty acids and measurements at different time intervals made as they relate to the above mentioned cardiovascular parameters. The knowledge gained will enable us to better understand the events contributing to the development of atherosclerosis and thromboembolytic disease.