MARINE LIPIDS & POST - PRANDIAL MEDIATORS IN MEN & WOMEN

海洋油对血管疾病的潜在健康益处是 深入调查，包括它们对血小板的影响， 内皮、巨噬细胞和脂蛋白(LP)。还有很棒的 在餐后血脂期间出现致动脉粥样硬化性脂蛋白的兴趣， 而海洋脂肪中的N-3脂肪酸(FA)对后处理有显著影响。 膳食脂血症现在得到了很好的证实。然而，目前还没有研究表明 明确了餐后脂蛋白、血管内皮和血液的相互作用 人体中的元素或摄取海油可能如何改变这种 流程。拟议研究的目的将是利用国家-- 最先进的GC/MS方法和给药定义的测试餐到10 男性脂蛋白加工过程中血小板/血管相互作用的研究 以及这种影响是如何由急性和慢性疾病改变的 摄取海洋油类；20评估N-3脂肪酸和α-脂肪酸的影响。 生育酚对餐后加工过程中氧化性脂蛋白生成的影响 由正常和动脉粥样硬化的受试者，以及通过 含氧脂质的胆汁进入胃肠道，可合并到 新生的乳杆菌，以及3)研究脂肪酸的转移， 甾醇、其含氧衍生物和α-生育酚对单核细胞的作用 在摄入海洋油或对照油后的餐后血脂期间， 以及海洋油和富含海洋油的低密度脂蛋白对人单核细胞的影响 和巨噬细胞胆固醇酯代谢，二十烷类化合物的合成和 趋化性。将使用生理学和药理学方法来 明确血小板/血管、血管/脂蛋白的重要方面 人类中脂蛋白/单核细胞的相互作用，比较男性的这些事件 和女性，并决定她们如何受到海洋石油的影响。通过做 因此，拟议的项目直接在体内解决了几个重要的 当前人类动脉粥样硬化形成理论的基础假设，因此 将更清楚地定义海洋石油对健康的潜在益处 西方社会。

The potential health benefits of marine oils in vascular disease are being intensively investigated, including their effects on platelets, endothelium, macrophages and lipoproteins (LP). There is also great interest in the appearance of atherogenic LP during post-prandial lipemia, and striking effects of the N-3 fatty acids (FA) in marine lipids on post- prandial lipemia are now well-established. However, no studies have yet defined the post-prandial interactions of LP, endothelium and blood elements in humans or how ingestion of marine oils might alter such processes. The object of the proposed research will be to utilize state- of-the-art GC/MS methods and administration of defined test meals to 10 study platelet/vascular interactions during lipoprotein processing in men and women, and how such effects are changed by the acute and chronic ingestion of marine oils; 20 assess the effects of N-3 FA and alpha- tocopherol on the generation of oxidized LP during post-prandial processing by normal and atherosclerotic subjects, as well as the delivery via the bile of oxygenated lipids to the G.I. tract that could be incorporated into nascent chylomicrons, and 3) investigate the transfer of fatty acids, sterols, their oxygenated derivatives, and alpha-tocopherol to monocytes during post-prandial lipemia following ingestion of marine or control oils, and the effects of marine oils and marine-oil enriched LP on human monocyte and macrophage cholesterol ester metabolism, eicosanoid synthesis and chemotaxis. Physiological and pharmacological approaches will be used to define important aspects of platelet/vascular, vascular/lipoprotein, and lipoprotein/monocyte interactions in humans, compare these events in men and women, and determine how they are influenced by marine oils. By doing so, the proposed project directly addresses in vivo several important hypotheses underlying current theories of human atherogenesis, and thereby will define more clearly the potential health benefits of marine oils in Western societies.