FINE STRUCTURAL PATHOLOGY OF MYELIN

研究了两种病毒的脱髓鞘和髓鞘再生机制 用电子显微镜诱发（SJL/Balb/c）F1小鼠脱髓鞘病 显微镜、免疫荧光和放射自显影。 实验 模型是由小鼠肝炎诱导的小鼠脑白质脊髓炎 病毒（MHV）或泰勒小鼠脑脊髓炎病毒（DAV）的DA株。 目的是：1）鉴定T淋巴细胞亚群， 不同疾病阶段的炎性脱髓鞘病变， 将它们的分布与过敏性 脑脊髓炎（EAE）。 2)测定脾细胞过敏性 细胞对小鼠脊髓匀浆（MSCH），髓鞘碱性蛋白 (MBP)在DAV诱导的脱髓鞘过程中灭活DAV。 3)在给药前和给药期间转移小鼠的淋巴结和脾细胞。 DAV感染后出现白色病变，并寻找 作为阳性对照， 转移患有EAE的小鼠的淋巴细胞。 4)研究 在植入产生免疫球蛋白的杂交瘤的小鼠中， 从MHV感染中恢复。 预计调查结果将 有助于了解人类感染后 脑脊髓炎和多发性硬化。

Mechanisms of demyelination and remyelination are studied in two virus induced demyelinating diseases in (SJL/Balb/c) F1 mice by means of electron microscopy, immunofluorescence and radioautography. The experimental models are murine leucoencephalomyelitis induced by either mouse hepatitis virus (MHV) or DA strain of Theiler's mouse encephalomyelitis virus (DAV). The objectives are to: 1) identify subpopulations of T-lymphocytes in the inflammatory demyelinating lesions at different stages of disease and compare their distribution to lesions produced by allergic encephalomyelitis (EAE). 2) Determine cellular hypersensitivity of spleen cells against mouse spinal cord homogenate (MSCH), myelin basic protein (MBP) and inactivated DAV during the course of DAV induced demyelination. 3) Transfer lymphnode and spleen cells from mice prior to and during the development of white matter lesions after infection with DAV and search for histologic lesions in recipient mice using as positive controls the transfer of lymphoid cells from mice afflicted with EAE. 4) Study remeylination in mice implanted with immunoglobulin producing hybridomas during recovery from MHV infection. The findings are expected to contribute to the understanding of the pathogenesis of human postinfectious encephalomyelitis and multiple sclerosis.