GENERATION AND REGENERATION OF AXONS

第一组拟议的实验旨在进行比较和对比 发生在再生系统中的突触发生，金鱼 视网膜顶盖系统，损伤诱导的重组发生在 在非再生系统中发芽的侧枝，大鼠的脚间 核(IPN)。具体问题涉及到与 管理重建特定数量和模式的因素 再生金鱼视网膜轴突与因子的联系 完整调控侧支发芽和反应性神经再支配 哺乳动物中枢神经系统损伤后的神经元。此信息在以下方面至关重要 为了预测所致损害的功能后果 成年中枢神经系统的突触发生。 第二个目标是确定哪些细胞过程区别于 视神经切断诱导大鼠视网膜神经节细胞的反应 通过植入技术延长轴突，从轴突切除但 不可再生的大鼠视网膜神经节细胞及其再生 金鱼视网膜神经节细胞。这些研究应该有助于 说明导致健壮和定向的过程 金鱼轴突切断后视神经轴突的生长及其影响因素 这种生长在被切断的哺乳动物的中央轴突中的正常失败。

The first set of proposed experiments is designed to compare and contrast synaptogenesis occurring in a regenerating system, the goldfish retinotectal system, with lesion induced reorganization occurring by collateral sprouting in a non-regenerating system, the rat interpeduncular nucleus (IPN). The specific issues are related to a comparison of the factors regulating reestablishment of specific numbers and patterns of connections by regenerating goldfish retinal axons with the factors regulating collateral sprouting and reactive reinnervation by intact neurons after lesions in mammalian CNS. This information is essential in order to predict the functional consequences of lesion induced synaptogenesis in the adult CNS. A second goal is to determine those cellular processes which distinguish the response of the rat retinal ganglion cell, axotomized and induced to elongate its axon via implantation techniques, from the axotomized but non-regenerating rat retinal ganglion cell and from the regenerating goldfish retinal ganglion cell. These studies should contribute to an elucidation of the processes which account for the robust and directed growth of the axotomized goldfish optic axons and those which account for the normal failure of this growth in the axotomized mammalian central axons.