ACTION OF MUSCLE TROPHIC FACTORS ON SPINAL MOTOR NEURONS

一种20000道尔顿的阳离子多肽已经被提纯到 新生大鼠骨骼肌提取物的同质性。这个 将纯化的多肽添加到解离培养物中 胚胎大鼠脊髓神经元促进发育的实验研究 胆碱乙酰转移酶和乙酰胆碱合成活性， 但不影响脊髓的整体代谢活动 文化。这种多肽代表了唯一的骨骼肌- 衍生神经营养因子纯化至均一，此外 NGF，它是唯一一种影响 中枢神经系统来源神经元的胆碱能发育。对雏鸡的治疗 卵子中的胚胎与部分纯化的多肽 正常细胞死亡期挽救运动神经元 可能已经死了。因此，这种神经营养多肽似乎是 对运动神经元的生存和发育很重要。我们的 目前的努力旨在进一步描述 该因子在体外和体内发育中的作用 通过直接应用和通过发展运动神经元 纯化因子的特异性抗体。

A 20,000 dalton cationic polypeptide has been purified to homogeneity from extracts of neonatal rat skeletal muscle. The addition of the purified peptide to dissociated cultures of embryonic rat spinal cord neurons enhances the development of choline acetyltransferase and acetylcholine synthesis activities, but does not affect the overall metabolic activity of spinal cord cultures. This polypeptide represents the only skeletal muscle- derived neurotrophic factor purified to homogeneity, and besides NGF, it is the only other neurotrophic factor which affects cholinergic development of CNS-derived neurons. Treatment of chick embryos in ovo with the partially purified peptide during the normal cell death period rescues motor neurons which otherwise would have died. Thus, this neurotrophic peptide appears to be important for the survival and development of motor neurons. Our present efforts are directed at further characterization of the effects of this factor on the in vitro and in vivo development of motor neurons by direct application and through the development of specific antibodies to the purified factor.