ANALYSIS OF FAST AXONAL TRANSPORT

视频增强显微技术的最新进展 使观察和记录快速轴突运输成为可能 大型无脊椎动物神经元轴浆内的管状细胞。在……里面 鱿鱼的巨大轴突，突出的轴浆几个小时都保持活跃， 为许多类型的实验提供了一个理想的范例。因此，现在就是这样 第一次有可能设计出旨在全面 分析快速轴突运输的机制。我们已经选出了六个 无脊椎动物神经制剂，每种都有一些特殊的特征 有利于本研究的开展。将使用avec方法来表征 这六种制剂的快速轴突运输和使用它们测试 关于快速轴突运输机制的若干假说。 实验方法将包括运动分析、药理学 实验，使用细胞骨架蛋白(或其衍生物)， 细胞骨架蛋白和机械酶的抗体，以及代谢 影响快速轴突运输的抑制剂。我们将努力 在足够温和的条件下破坏和分裂轴浆 运动性可以通过单一的分数来保持，也可以恢复到混合的 两个或更多分数。最终的目标是了解 快速轴突运输。

The recent development of the AVEC methods of video-enhanced microscopy have made it possible to observe and record fast axonal transport of tubulovesicular elements in the axoplasm of large invertebrate neurons. In the giant axon of the squid, extruded axoplasm remains active for hours, making an ideal paradigm for many types of experiments. Thus it is now possible for the first time to design experiments aimed at a comprehensive analysis of the mechanisms of fast axonal transport. We have selected six invertebrate nerve preparations, each having some special features advantageous to this study. The AVEC methods will be used to characterize fast axonal transport in these six preparations and using them test a number of hypotheses regarding the mechanisms of fast axonal transport. Experimental approaches will include motion analysis, pharmacological experiments, the use of cytoskeletal proteins (or derivatives therof), antibodies to cytoskeletal proteins and mechanoenzymes, and metabolic inhibitors to influence fast axonal transport. Efforts will be made to disrupt and fractionate axoplasm under conditions sufficiently mild that motility can be retained by a single fraction or restored to a mixture of two or more fractions. The ultimate goal is to understand the process of fast axonal transport.