BILIRUBIN TOXICITY IN THE AUDITORY SYSTEM

胆红素脑病，胆红素中毒的结果 大脑，会导致大脑损伤和耳聋。其发病机制及发病机制 胆红素致听神经功能障碍部位(S) 脑病仍然是未知和有争议的，尽管 几十年的研究。脑干听觉诱发电位， 组织学和生化分析将应用于Gunn大鼠 胆红素脑病模型的听觉定位(S) 功能障碍，其时间进程，与发育的相互作用，以及 潜在的可逆转性。 该项目将使用脑干听觉诱发电位来评估神经生理变化 急性胆红素中毒后不久发生的。脑干听觉诱发电位 研究结果将与对游离和蛋白质的测量结果进行比较- 结合血胆红素，然后进行组织学检查。研究地点： 不同的年龄将检查增加的脆弱性 未成熟的中枢神经系统对胆红素的毒性。 旨在降低胆红素毒性的干预措施将探索 病理过程可逆性的时间限制。 在相关研究中，该项目将本地化的具体地点(S) 胆红素致听觉神经系统功能障碍。BAEP和 将进行组织学比较，以验证耳蜗和 听觉脑干核团。这些发现还将与以下方面相关 前面描述的生化研究。 由此产生的多学科方法预计将提供 对本病发病机制及其影响的新认识 在听觉系统上。以及对情结的理解 电生理学、解剖学和神经生物学之间的关系 生化过程应该导致改进的非侵入性 预测程序(例如脑干听觉诱发电位和血液测试) 人类新生儿的神经学和耳科后遗症。

Bilirubin encephalopathy, the result of bilirubin toxicity to the brain, causes brain damage and deafness. The pathogenesis and site(s) of auditory nervous system dysfunction in bilirubin encephalopathy are still unknown and controversial despite decades of study. Brainstem auditory evoked potentials (BAEPs), histology, and biochemical assays will be applied to the Gunn rat model of bilirubin encephalopathy to define the site(s) of auditory dysfunction, its time course, interaction with development, and potential for reversibility. The project will use BAEPs to assess neurophysiological changes occurring soon after acute exposure to bilirubin toxicity. BAEP findings will be compared to measurements of free and protein- bound blood bilirubin, and then to histological findings. Studies at different ages will examine the increased vulnerability of the immature central nervous system to bilirubin toxicity. Interventions aimed at decreasing bilirubin toxicity will explore the time constraints of reversibility of the pathological process. In related studies, the project will localize the specific site(s) of bilirubin induced auditory nervous system dysfunction. BAEPs and histology will be compared to verify the status of the cochlea and the auditory brainstem nuclei. The findings will also be related to the previously described biochemical studies. The resulting multidisciplinary approach is expected to provide new insights into the pathogenesis of this disorder and its effects on the auditory system. And understanding of the complex relationships between electrophysiological, anatomical and biochemical processes should lead to improved noninvasive procedures (e.g. BAEPs and blood tests) for predicting neurological and otological sequelae in human newborns.