NERVE GROWTH CONE LOCOMOTION

神经生长锥体在发育过程中的运动 再生被认为是引导增长的重要因素 轴突沿着正确的路径到达它的目标。这个过程是 对正常发育或功能恢复至关重要 神经损伤。神经生长锥运动是一个复杂的过程。 在细胞培养中研究得最好的现象。至 了解神经生长的内在机制 锥体运动，将采取多学科方法。大鼠 在细胞培养中生长的颈上神经节神经元将 使用。假设肌动蛋白和肌球蛋白 参与生长锥运动，免疫电子显微镜会 寻求确定生长锥的分子组织 肌球蛋白和肌动蛋白。微量注射抗肿瘤单抗 肌球蛋白将被用来确定肌球蛋白在 产生收缩张力，使生长锥体移动 穿过底层。生长锥的荧光比成像 然后使用特定指示剂Fura-2进行钙化 确定钙在运动控制中的作用 机制。这些实验将提供一个基本的框架 以了解引导运动的机制。

The locomotion of the nerve growth cone during development or regeneration is thought to be important for steering a growing neurite down the correct pathway to its target. This process is essential for normal development or functional recovery aftter nerve damage. Nerve growth cone locomotion is a complex phenomenon which has been best studied in cell culture. To understand the underlyng mechanism responsible for nerve growth cone locomotion, a multidisciplinary approach will be taken. Rat superior cervical ganglion neurons grown in cell culture will be used. Working under the assumption that actin and myosin are involved in growth cone motility, immunoelectron microscopy will seek to determine the molecular organization of growth cone myosin and actin. Microinjection of monoclonal antibodies to myosin will be used to determine the role of myosin in the production of contractile tension that moves the growth cone across a substratum. Fluorescence ratio imaging of growth cone calcium using the specific indicator fura-2 will then be done to determine the role calcium plays in the control of the moility mechanism. These experiments will provide a basic framework for understanding the mechanism of guided locomotion.