NERVE GROWTH CONE LOCOMOTION

神经生长锥在发育过程中的运动， 再生被认为是重要的， 沿着正确的路径到达目标 这个过程是 对正常发育或功能恢复至关重要 神经损伤 神经生长锥运动是一个复杂的 这种现象在细胞培养中得到了最好的研究。 到 了解神经生长的基本机制 锥运动，将采取多学科的方法。 大鼠 在细胞培养物中生长的上级颈神经节神经元将 采用 假设肌动蛋白和肌球蛋白 参与生长锥运动，免疫电镜将 试图确定生长锥的分子结构 肌球蛋白和肌动蛋白。 显微注射单克隆抗体 肌球蛋白将被用来确定肌球蛋白的作用， 产生收缩张力，使生长锥移动 穿过底层 生长锥的荧光比率成像 然后使用特定指示剂Fura-2进行钙测定， 确定钙在运动控制中的作用 机制 这些实验将提供一个基本框架 来理解引导运动的机制。

The locomotion of the nerve growth cone during development or regeneration is thought to be important for steering a growing neurite down the correct pathway to its target. This process is essential for normal development or functional recovery aftter nerve damage. Nerve growth cone locomotion is a complex phenomenon which has been best studied in cell culture. To understand the underlyng mechanism responsible for nerve growth cone locomotion, a multidisciplinary approach will be taken. Rat superior cervical ganglion neurons grown in cell culture will be used. Working under the assumption that actin and myosin are involved in growth cone motility, immunoelectron microscopy will seek to determine the molecular organization of growth cone myosin and actin. Microinjection of monoclonal antibodies to myosin will be used to determine the role of myosin in the production of contractile tension that moves the growth cone across a substratum. Fluorescence ratio imaging of growth cone calcium using the specific indicator fura-2 will then be done to determine the role calcium plays in the control of the moility mechanism. These experiments will provide a basic framework for understanding the mechanism of guided locomotion.