LUTEAL FUNCTION--ROLES OF GROWTH FACTORS AND ONCOGENES

这项研究的广泛和长期目标是进一步推动我们的 通过调查了解黄体的内分泌学 遗传正常细胞生长过程中的调控机制 生理系统。然而，原癌基因的表达也 与不受控制的生长有关，即乳房、卵巢、结肠、 人类的膀胱和胃肿瘤。在内分泌系统中， 哺乳动物在周期调节和生育控制中的作用。因此，它是一种 理想的器官，不仅用于研究正常组织中的细胞增殖， 但要研究原癌基因的表达如何与 生长因子和激素在调节黄体功能中的作用。这个 奶牛的黄体将被用来鉴定特定的原癌基因 它们在黄体发生过程中被开启和关闭。 相对表达水平将与黄体大小相关，并且 组织和血浆中的孕酮浓度。原癌基因 转录水平的表达将通过斑点印迹和 Northern-Blot杂交。黄体酮浓度将会是 由RIA测定。使用同样的分析技术，监管机构 促黄体生成素和生长因子对原癌基因表达的影响 早期黄体将在长期培养系统中进行研究。它 预计这些结果将在 黄体的生殖生理和基因表达，并将 提供对正常生长发育调节的洞察力 组织。

The broad, long-term objective of this study is to further our understanding of the endocrinology of the corpus luteum by investigating regulatory mechanisms at the genetic normal cellular growth processes in physiological systems. However, expression of proto-oncogenes has also been associated with uncontrolled growth, i.e. breast, ovarian, colon, bladder and stomach tumors in humans. Within the endocrine system, the mammalian role in cycle regulation and fertility control. Thus, it is an ideal organ, not only to study cellular proliferation in a normal tissue, but to study how the expression of proto-oncogenes is associated with the action of growth factors and hormones in regulating luteal function. The corpus luteum of the cow will be used to identify specific proto-oncogenes that are turned on and off during the genesis of the corpus luteum. Relative levels of expression will be correlated to luteal size, and progesterone concentrations in tissue and plasma. Proto-oncogene expression at the transcriptional level will be detected by dot-blot and Northern-blot hybridizations. Progesterone concentrations will be determined by RIA. Using these same analytical techniques, the regulation of proto-oncogene expression by luteinizing hormone and growth factors in the early corpus luteum will be studied in a long-term culture system. It is anticipated that these results will contribute new information in the reproductive physiology of the corpus luteum and gene expression, and will provide insight into the regulation of growth and development in normal tissue.