ROLE OF REGULATORY PROTEINS IN ORAL HERPES REACTIVATION

描述(改编自《调查者摘要》)：长期 这项建议的目的是了解负责 重新激活潜伏的口腔单纯疱疹病毒I型(HSV-1)。建议数 研究基于这样的假设，即紫外线诱导的细胞 蛋白质通过结合潜伏病毒的启动子来增强病毒的重新激活 相关转录本基因，唯一的转录活性部位 延迟。具体目的是：(1)识别疱疹病毒的DNA区域 通过迁移率与紫外线诱导的细胞蛋白结合的单纯疱疹病毒1型 Shift分析；(2)确定特定靶点的DNA结合部位 1通过DNase I足迹；以及(3)鉴定、分离和测序 编码特定DNA结合蛋白的细胞基因 目标1。

DESCRIPTION (adapted from the Investigator's Abstract): The long term objective of this proposal is to understand the mechanism responsible for reactivating latent oral herpes simplex virus type I (HSV-1). The proposed studies are based on the hypothesis that ultraviolet light-induced cell proteins enhance virus reactivation by binding the promoter of the latent- associated transcripts gene, the only transcriptionally active site during latency. The specific aims are: (1) to identify the DNA region of herpes simplex virus type 1 that binds UV-induced cell proteins using mobility shift assay; (2) to delimit the DNA binding site identified in Specific Aim 1 by DNase I footprinting; and (3) to identify, isolate, and sequence the cellular gene that encodes the DNA binding protein identified in Specific Aim 1.