CAN THE RAPD TECHNIQUE BE USED FOR GENETIC MAPPING

RAPD技术采用聚合酶链反应（PCR）， 随机引物来鉴定不同品系中的多态性。 因为PCR 更简单、更快、更容易自动化，而且可能比 其他遗传鉴定方法（如RFLP和同工酶）， 对绘制人类基因图谱有着巨大的潜在效用。 然而，理论上 可以提出这样的论点，即RAPD多态性可能不映射到固定的 地点 本项目的目的是通过以下方式来检验这一论点： 对4个不同玉米品系的RAPD多态性进行了定位。 这些玉米 品系被使用是因为从它们衍生的重组-近交系 使多态性的快速作图成为可能。为了让这项技术 对于作图有用的是，多态性条带应在同一位置作图， 所有线路。 其他实验将探索竞争的影响 在F1杂种DNA扩增过程中多态性位点之间 以及多态性条带与其他物种杂交的能力 扩增序列

The RAPD technique uses polymerase chain reaction (PCR) with single short random primers to identify polymorphisms in different lines. Because PCR is simpler, faster, easier to automate, and potentially cheaper than other genetic identification methods (such as RFLPs and isozymes), it has great potential utility for mapping human genes. However, a theoretical argument can be made that RAPD polymorphisms may not map to fixed locations. The purpose of this project is to test this argument by mapping RAPD polymorphisms in four different maize lines. These maize lines are being used because recombinant-inbred lines derived from them make the rapid mapping of polymorphisms possible. For the technique to be useful for mapping, polymorphic bands should map to the same location in all lines. Other experiments will explore the effects of competition between polymorphic sites during the amplification of DNA from Fl hybrids as well as the ability of polymorphic bands to cross-hybridize with other amplified sequences.