FUNCTION OF E COLI 4.5S RNA

蛋白质合成的控制是其基本机制之一 参与调节细胞的生长。 新发现 平移装置的部件必须完全 调查，以确定其功能和重要性， 过程 E.大肠杆菌，一种新发现的 蛋白质合成的起始阶段涉及到蛋白质合成的起始阶段。 本项目是第一个详细研究这一功能的 在分子水平上。 通过基因构建， 其中4.5S RNA的合成是可调控的。 当合成 关闭，正常的细胞生理机能被破坏，最终 导致细胞死亡。 蛋白质合成的起始是 第一个可观察到的代谢过程受到影响。 这个项目 将详细调查启动中的缺陷，并确定 需要4.5S RNA的确切反应。 此外一个 已提出的4.5S RNA功能的特定模型 将直接测试。

The control of protein synthesis is one of the basic mechanisms involved in regulating the growth of cells. Newly discovered components of the translational apparatus must be fully investigated to establish their function and importance in this process. The 4.5S RNA of E. coli, one such newly discovered component, is involved in the initiation phase of protein synthesis. This project is the first detailed study of the function of this molecule on a molecular level. Through genetic construction, a bacterial strain has been made in which synthesis of 4.5S RNA can be regulated. When synthesis is turned off, normal cellular physiology is disrupted, eventually leading to death of the cell. Initiation of protein synthesis is the first observable metabolic process to be affected. This project will investigate in detail the defect in initiation, and determine the exact reaction which requires 4.5S RNA. In addition, one specific model which has been proposed for 4.5S RNA function will be directly tested.