A NEW TOXIC VIRULENCE FACTOR OF AEROMONAS HYDROPHILA

这项研究的主要目的是确定一个 一种新发现的霍乱毒素交叉反应因子 嗜水气单胞菌介导的疾病。 相关毒力因子 名为.文献中报道的Escherichia并不清楚 定义了 A.霍乱弧菌的近亲， 被认为是人类重要的病原体。 这种生物能够 导致多种疾病，包括急性细菌性腹泻， 败血症、脑膜炎、心内膜炎、角膜溃疡、腹膜炎，以及 伤口感染 即使在健康的， 有免疫能力的人。 疾病的症状产生于 芳香单胞菌感染部分与有毒的 胞外产物 我们发现A.大肠杆菌菌株SSU， 在酪蛋白氨基酸-酵母提取物（CYE）培养基中生长，可以产生高水平的 与霍乱肠毒素交叉反应的有毒细胞外产物 通过酶联免疫吸附测定（ELISA）测定。 我们有 将这种胞外产物称为“霍乱毒素交叉反应因子” 直到完成进一步的表征。 同样的培养滤液 含有这种霍乱毒素交叉反应因子会对Vero细胞造成损害 （绿色猴肾）细胞膜和兔红细胞裂解。 当将这种滤液静脉注射到小鼠体内时， 展出。 我们将提纯并鉴定这种霍乱毒素 交叉反应因子的抗原性、生物学和生物化学。 我们 实验室将研究这种细胞外的作用机制， 因子 此外，编码基因的遗传信息类型 将探索这种毒力因子的合成。 理化 有关霍乱毒素交叉反应因子的研究将会展开。 这 霍乱毒素交叉反应的胞外产物可能是 气单胞菌外毒素和细胞毒素在CYE培养基中结合， 具有肠毒性和细胞毒性的较大复杂分子 活动 本提案中描述了实验来调查这一点 假说. 涉及这种潜在毒性的拟议研究 这一因素将提高我们对气单胞菌机制的理解 尿道感染。

The main objective of this proposed research is to determine the role of a newly described cholera toxin crossreactive factor in the pathogenesis of Aeromonas hydrophila mediated diseases. The virulence factors associated with A. hydrophila reported in the literature have not been clearly defined. A. hydrophila, a close relative to Vibrio cholera, has recently been recognized as an important human pathogen. This organism is capable of causing a variety of diseases, including acute bacterial diarrhea, septecemia, meningitis, endocarditis, corneal ulcers, peritonitis, and wound infections. Fatalities have occurred even in healthy, immunologically competent persons. The symptoms of disease produced during Aromonas infections are in part related to the elaboration of toxic extracellular products. We have discovered that A. hydrophila strain SSU, grown in Casamino acid-yeast extract (CYE) medium, can produce high levels of a toxic extracellular product that crossreacts with cholera enterotoxin as determined by the enzyme-linked immunosorbent assay (ELISA). We have termed this extracellular product "cholera toxin crossreactive factor" until further characterization is accomplished. The same culture filtrate containing this cholera toxin crossreactive factor causes damage to Vero (green monkey kidney) cell membranes and lysis of rabbit red blood cells. When this filtrate was injected intravenously into mice, lethal effects are exhibited. We will purify and characterize this cholera toxin crossreactive factor antigenically, biologically, and biochemically. Our laboratory will investigate the mechanism of action of this extracellular factor. In addition, the type of genetic information that codes for the synthesis of this virulence factor will be explored. Physicochemical studies of this cholera toxin crossreactive factor will be initiated. This cholera toxin crossreactive extracellular product may be the result of Aeromonas exterotoxin and cytotoxin combining in the CYE medium to make a larger complex molecule possessing both enterotoxic and cytotoxic activities. Experiments are described in this proposal to investigate this hypothesis. The proposed research involving this potential virulence factor should improve our understanding of the mechanisms of Aeromonas hydrophila infections.