SECRETION OF GH AND PRL FROM INDIVIDUAL GH3 CELLS

GH 3细胞系已被广泛用于研究催乳素（PRL）和 生长激素（GH）分泌。 这些研究提供的信息 深入了解正常垂体细胞分泌激素的过程。 GH 3细胞可以在培养物中维持长时间，而不需要细胞内的细胞毒性。 失去分泌GH和PRL的能力。 同样，这些细胞的培养 不含其他类型的分泌物，这些分泌物存在于 正常垂体细胞 这些和其他特性使得实验 太难或不可能使用 正常垂体细胞 此外，有人建议， GH培养物中的单个细胞分泌GH和PRL。 这是非常 重要的是，根据最近的观察， GH和PRL也存在于正常垂体细胞的培养物中。 因此，在本发明中， GH 3培养物提供了一种模型，可用于评估 这两种激素分泌的不同细胞类型。 最近 我们实验室的实验表明， 其间接改变了整个GH 3培养物的GH和PRL分泌， 细胞还诱导GH和PRL比例的相互转移 培养中的分泌细胞。 这些发现表明， GH和/或PRL分泌细胞类型的相互转化可能在 调节GH 3培养物中的激素分泌，并提高 在生理学上可能涉及类似的过程， 调节GH和PRL分泌。 因此，本研究的目的 鉴定GH 3培养物中PRL和/或GH分泌物的类型， 确定两种细胞类型的相互转化是否有助于 激素分泌的变化。 我计划通过以下方式实现这些目标： 使用反向溶血空斑试验，该试验能够鉴定 从单个细胞中分泌激素以确定激素的类型 文化中的秘书 然后我会在慢性期监测单个细胞 激素治疗以确定一种细胞类型的相互转化 另一个发生。 拟议的研究将为一些问题提供答案。 关于改变细胞类型参与 调节GH和PRL分泌。 此外，这些信息可以用于 为未来的研究提供更有洞察力的设计， 了解垂体异常的原因和治疗，如 如微腺瘤，或治疗和控制这种病理性 乳腺溢液-闭经或侏儒症等病症。

The GH3 cell line has been used extensively to study prolactin (PRL) and growth hormone (GH) secretion. Information from these studies has provided insight into the process of hormone secretion from normal pituitary cells. GH3 cells can be maintained in culture for long periods of time without losing the capacity to secrete GH and PRL. Also, cultures of these cells do not contain other types of secretors that are found in preparations of normal pituitary cells. These and other characteristics enable experiments to be performed that are too difficult or impossible to conduct using normal pituitary cells. Additionally, it has been suggested that individual cells in GH cultures secrete both GH and PRL. This is very important in light of the recent observation that cells which secrete both GH and PRL are also present in cultures of normal pituitary cells. Thus, GH3 cultures provide a model that can be used to assess the importance of different cell types in the secretion of these two hormones. Recent experiments in our laboratory demonstrate that chronic hormonal treatment which reciprocally alters GH and PRL secretion from entire cultures of GH3 cells also induces a reciprocal shift in the proportion of GH and PRL secreting cells in culture. These findings indicate that an interconversion of GH and/or PRL secreting cell types may be important in the modulation of hormone secretion in GH3 cultures and raise the possibility that a similar process may be involved in the physiological regulation of GH and PRL secretion. Therefore, the purpose of this study is to identify the types of PRL and/or GH secretors in GH3 cultures and to determine whether an interconversion of two cell types may contribute to changes in hormone secretion. I plan to accomplish these objectives by using the reverse hemolytic plaque assay, which enables identification of hormone secretion from individual cells to establish the types of hormone secretors in culture. I will then monitor individual cells during chronic hormonal treatment to determine whether an interconversion of one cell type to another occurs. The proposed studies will provide an answer to some very basic questions about the involvement of changing cell types in the modulation of GH and PRL secretion. Moreover, this information can be used for more insightful design of future studies that may lead to eventual understanding of the cause and treatment of pituitary abnormalities, such as microadenmas, or the treatment and control of such pathological conditions such as galactorrhea-amenorrhea or dwarfism.