STRUCTURAL MOLECULE INTERACTION OF LENS CELL-LENS CAPSUL

设计了一个监测正常牙体粘附性的模型系统 牛晶状体上皮细胞到晶状体囊膜，天然基底膜 在活体中这些细胞就在上面休息。该系统提供了一个良好的 研究上皮细胞相关决定因素的模型 细胞-基底膜相互作用。结合使用此方法， 通过以下过程对晶状体胶囊进行有限增溶 使用溶剂提取而不是酶处理，这是可能的 目的：获得沙门氏菌特定基底膜糖蛋白的亚组分。 镜片胶囊。然后人们可以评估这些非胶原蛋白的作用 晶状体细胞-晶状体囊内细胞黏附和扩散的蛋白质 依恋试验。 细胞在天然基底膜上的黏附和扩散 晶状体前囊膜上皮细胞的研究 从原代培养、早期继代培养和后期传代获得 培养物，以及正常的人成纤维细胞和仓鼠气管 上皮细胞。测试基片将包括本地晶状体胶囊， 残留的晶状体囊，变性的I型和IV型胶原蛋白和水化的 基底膜存在或不存在时的胶原凝胶 小数。细胞黏附和扩散的生物学要求， 将进行研究，包括晶状体胶囊的生化特性 天然基底膜的结构组成及其影响 对细胞超微结构及基底膜表达的影响 贴壁细胞的成分。关于结构部件的知识 晶状体囊膜及其对晶状体上皮细胞的影响 正常附着在细胞上的细胞将促进我们对细胞的理解 增殖和分化，并提出防止或逆转的方法 糖尿病和白内障的基底膜相关并发症 队形。

A model system has been devised which monitors the adhesion of normal bovine lens epithelial cells to lens capsule, the native basement membrane upon which these cells rest in vivo. This system provides a favorable model for investigating the determinants involved in epithelial cell-basement membrane interactions. Using this method, in conjunction with a limited solubilization of the lens capsule by a procedure which employs solvent extraction rather than enzymatic treatment, it is possible to obtain subfractions of specific basement membrane glycoproteins of the lens capsule. One can then evaluate the role of these non-collagenous proteins in cellular adhesion and spreading in the lens cell-lens capsule attachment assay. Cellular adhesion and spreading on native basement membrane will be investigated using the epithelial cells of the anterior lens capsule obtained from primary cultures, early secondary cultures, and late passage cultures, as well as normal human fibroblasts and hamster tracheal epithelial cells. Test substrata will include native lens capsules, "residual" lens capsules, denatured types I and IV collagen and hydrated collagen gels in the presence or absence of basement membrane subfractions. The biological requirements for cell adhesion and spreading, will be studied, including a biochemical characterization of lens capsule structural components and the influence that native basement membrane exerts on cell ultrastructure and the expression of basement membrane constituents of adherent cells. A knowledge of the structural components of the lens capsule and the influence they may exert on lens epithelial cells normally attached thereto will advance our understanding of cell proliferation and differentiation and suggest ways to prevent or reverse the basement membrane related complications of diabetes and cataract formation.