STRUCTURAL MOLECULE INTERACTION OF LENS CELL-LENS CAPSUL

设计了一个模型系统，它监测正常的粘附力， 牛透镜上皮细胞至透镜囊，即天然基底膜 这些细胞在体内停留在其上。 该系统提供了一个有利的 研究上皮细胞相关决定因素的模型 细胞-基底膜相互作用 使用这种方法， 通过一种方法使透镜囊有限溶解，该方法 采用溶剂提取而不是酶处理， 以获得特异性基底膜糖蛋白的亚组分， 透镜囊。 然后可以评估这些非胶原蛋白的作用， 蛋白质在透镜细胞-透镜囊中的细胞粘附和扩散 附着试验 细胞在基底膜上的粘附和铺展将被破坏。 使用前透镜囊的上皮细胞进行研究 从原代培养物、早期传代培养物和晚期传代培养物中获得 培养物，以及正常人成纤维细胞和仓鼠气管 上皮细胞 试验基质将包括天然透镜囊， “残留”透镜囊、变性I型和IV型胶原蛋白和水合 胶原凝胶在有或无基底膜的情况下 亚分数 细胞粘附和扩散的生物学要求， 将进行研究，包括透镜囊的生化表征 结构成分和影响， 影响细胞超微结构和基底膜的表达 贴壁细胞的成分。 结构组成的知识 以及它们可能对透镜上皮细胞产生的影响 正常情况下附着于其上的细胞将促进我们对细胞的理解， 增殖和分化，并提出防止或逆转 糖尿病和白内障的基底膜相关并发症 阵

A model system has been devised which monitors the adhesion of normal bovine lens epithelial cells to lens capsule, the native basement membrane upon which these cells rest in vivo. This system provides a favorable model for investigating the determinants involved in epithelial cell-basement membrane interactions. Using this method, in conjunction with a limited solubilization of the lens capsule by a procedure which employs solvent extraction rather than enzymatic treatment, it is possible to obtain subfractions of specific basement membrane glycoproteins of the lens capsule. One can then evaluate the role of these non-collagenous proteins in cellular adhesion and spreading in the lens cell-lens capsule attachment assay. Cellular adhesion and spreading on native basement membrane will be investigated using the epithelial cells of the anterior lens capsule obtained from primary cultures, early secondary cultures, and late passage cultures, as well as normal human fibroblasts and hamster tracheal epithelial cells. Test substrata will include native lens capsules, "residual" lens capsules, denatured types I and IV collagen and hydrated collagen gels in the presence or absence of basement membrane subfractions. The biological requirements for cell adhesion and spreading, will be studied, including a biochemical characterization of lens capsule structural components and the influence that native basement membrane exerts on cell ultrastructure and the expression of basement membrane constituents of adherent cells. A knowledge of the structural components of the lens capsule and the influence they may exert on lens epithelial cells normally attached thereto will advance our understanding of cell proliferation and differentiation and suggest ways to prevent or reverse the basement membrane related complications of diabetes and cataract formation.