PATHOBIOLOGY OF CULTURED GLOMERULAR EPITHELIAL CELLS

这个项目的目的是研究培养的人对 肾小球内脏上皮细胞向非免疫性和 免疫损伤。肾小球上皮细胞似乎扮演着一个 嘌呤霉素在氨基核苷肾病和Heymann中的作用 大鼠肾炎模型。这些实验模型非常类似于 人类肾小球疾病-微小病变型肾病综合征 膜性肾病。我们之前已经 表明这些受体携带C3受体，C3的抗原 他们表面的海曼肾炎和他们合成的 硫酸乙酰肝素蛋白多糖。唾液酸残留物，富含 沉积在这些上皮细胞上，以及肾小球肝素 据说硫酸盐参与了肾小球屏障功能 防止蛋白质流失。包括膜在内的补体系统 已知的攻击综合体与 包括Heymann在内的许多肾小球疾病的发病机制 肾炎。利用上述属性，具体而言，我们 建议研究以下内容： 一、嘌呤霉素氨基核苷的作用(非免疫性损伤) Heymann肾炎相关抗原、硫酸乙酰肝素和唾液酸的研究 肾小球上皮细胞的酸含量。 A.与Heymann肾炎相关抗体的结合作用 定位于肾小球上皮细胞的抗原(免疫损伤) 对补体C3受体表达、硫酸乙酰肝素含量和 唾液酸。 B.结合C3配体、膜攻击的影响 内容物复杂、单独或组合(免疫损伤) 硫酸乙酰肝素和唾液酸。 所采用的方法将包括肾小球细胞培养 定量免疫过氧化物酶上皮细胞，标准玫瑰花环 利用绵羊红细胞作为指示粒子的技术。在……里面 免疫过氧化物酶反应，高度纯化，很好地描述 将使用特定的抗体。标准参数和 非参数统计方法将被用于分析 结果。我们的研究结果将有助于我们更好地理解 两种实验性肾脏的肾小球内脏上皮细胞 作为普通人类原型的疾病模型 肾小球疾病。

The aim of this project is to study the response of cultured glomerular visceral epithelial cells to nonimmunologic and immunologic injury. Glomerular epithelial cells appear to play a role in puromycin aminonucleoside nephrosis and Heymann nephritis in rat. These experimental models closely resemble human glomerular diseases - minimal change nephrotic syndrome and membranous nephropathy, respectively. We have previously shown that these receptors bear receptors for C3, antigens of Heymann nephritis on their surface and that they synthesize heparan sulfate proteoglycan. Sialic acid residues, richly deposited on these epithelial cells, and, glomerular heparan sulfate are said to be involved in glomerular barrier function against protein loss. Complement system including the membrane attack complex is known to be prominently involved in pathogenesis of many glomerular diseases including Heymann nephritis. Employing the above properties, specifically, we propose to study the following: I. Effect of puromycin aminonucleoside (nonimmunologic injury) on Heymann nephritis related antigen, heparan sulfate and sialic acid contents of glomerular epithelial cells. II. A. Effect of binding of antibody to Heymann nephritis related antigen located on glomerular epithelial cells (immunologic injury) on expression of C3 receptors, content of heparan sulfate and sialic acid. B. Effects of binding of C3 ligands, membrane attack complex, alone or in combination (immunologic injury) on content of heparan sulfate and sialic acid. Methods employed will consist of cell culture of glomerular epithelial cells, quantitative immunoperoxidase, standard rosette techniques using sheep erythrocytes as indicator particles. In immunoperoxidase reactions, highly purified, well described specific antibodies will be used. Standard parametric and nonparametric statistical methods will be used in analysis of results. Our results will advance understanding the role of glomerular visceral epithelial cells in the two experimental renal disease models which are prototypes of common human glomerulopathies.