REGENERATIVE POTENTIAL OF MUSCLE SPINDLES AFTER TRAUMA

我们计划研究哺乳动物肌梭的潜力 骨骼肌损伤后的修复。自动嫁接的 (移植)大鼠比目鱼肌作为实验模型。 创伤后肌肉再生和修复。我们的目标是探索 移植肌肉中肌梭的来源和形成 建立再生纺锤体的神经支配结构和模式。 一个重要的目标是确定纺锤体是否会重新形成。至 回答这个问题，我们将移植二腹肌，一块缺乏 纺锤，进入以前由含有纺锤的肌肉(A)占据的位置 有可能形成纺锤体的地点)。另一个目标是 评估感觉神经纤维和运动神经纤维在脑内的作用 确定再生主轴的结构。为此，我们 应检查在缺失的情况下再生的肌肉纺锤体 感觉或运动神经支配。运动神经轴突的神经营养作用 保险丝内纤维的性能将不受 通过比较在某个部位再生的肌梭的活动度 没有运动神经的和那些在静止状态下再生的， 运动神经支配的肌肉到正常大鼠的纺锤波。最后，我们将 确定再生肌肉中的纺锤体是否具有形态 特性以及运动和感觉神经支配，这将使 让他们以正常的方式运作。 这项研究将使用连续切片组织学和组织化学 技术，使用光学和电子显微镜。冰冻组织 将被染色以确定融合内肌的类型 纤维，以及感觉神经和运动神经。嵌在树脂中的肌肉 将在连续切片中染色，以允许重建分支 感觉轴突和运动轴突入纺锤的模式及检查 神经末梢的形式。这些技术允许详细地 正常人和正常人纺锤体结构的定量比较 再生的肌肉。 再生肌移植物中肌梭的研究 有助于了解肌肉移植或受伤的情况 重新设立为职能实体。

We plan to examine the potential of mammalian muscle spindles for restoration following skeletal muscle injury. The autografted (transplanted) rat soleus muscle is used as an experimental model of post-traumatic muscle regeneration and repair. Our aim is to explore the origin and formation of muscle spindles in the grafted muscles, and to establish the structure and patterns of innervation of regenerated spindles. An important goal is to determine whether spindles will form anew. To answer this question, we shall graft the digastric, a muscle devoid of spindles, into a site formerly occupied by a spindle-containing muscle (a site with potential for the formation of spindles). Another objective is to assess the respective roles of sensory and motor nerve fibers in determining the structure of regenerated spindles. For this reason, we shall examine spindles in muscles that have regenerated in the absence of sensory or motor innervation. Neurotrophic influences of motor axons on properties of intrafusal fibers will be separated from influences of activity by comparison of muscle spindles that have regenerated in a site devoid of motor innervation and those that have regenerated in quiescent, motor-innervated muscles to normal rat spindles. Lastly, we shall determine whether spindles in the regenerated muscle have morphological properties and motor and sensory innervation of the kind that would allow them to function in a normal manner. The study will use serial-sectioning histological and histochemical techniques, employing both light and electron microscope. Frozen tissues will be stained for enzymes which delineate types of intrafusal muscle fiber, as well as sensory and motor innervation. Muscles embedded in resin will be stained in serial sections to allow reconstructions of branching patterns of sensory and motor axons entering the spindles and examination of the form of nerve terminals. These techniques permit detailed quantitative comparison of the structure of spindles in normal and regenerated muscles. Investigation of muscle spindles in regenerating muscle grafts will contribute to the understanding of how transplanted or injured muscles are re-established as functional entities.