LIPID INCORPORATED SERUM-FREE MEDIUM

该项目的长期目标是设计一种无血清 用于哺乳动物细胞体外最佳生长的化学成分确定的培养基。 该提案的具体目的是通过非常规方法， 载体蛋白、脂肪酸、α-硫辛酸、脂溶性 维生素和胆固醇在现有的无血清培养基， 培养基（CEM）。 除了激素、生长和附着因子外， 是最佳生长和最终产品生产的重要物质 哺乳动物细胞体外培养。 预计在I期研究期间， 将开发将脂肪酸，脂溶性维生素， α-硫辛酸和胆固醇转化为CEM而不使用载体 proteins. 第二阶段的研究将集中在进一步改善CEM， 将有机和水溶性激素和生长因子掺入到 CEM。 一种无血清CEM，将优化细胞在体外的生长 将在研究和生物技术实验室中得到广泛应用。 这种产品的商业价值将是巨大的。

The long term objective of this project is to design a serum-free chemically defined medium for optimum growth of mammalian cells in vitro. The specific aim of this proposal is to solubilize, by means other than the use of carrier proteins, fatty acids, alpha-lipoic acid, fat soluble vitamins and cholesterol in an existent serum-free medium, Chee's Essential Medium (CEM). Besides hormones, growth and attachment factors, lipids may be important substances for optimum growth and production of end products by mammalian cells in vitro. It is expected that during Phase I studies, methods will be developed to incorporate fatty acids, fat-soluble vitamins, alpha-lipoic acid and cholesterol into CEM without the use of carrier proteins. Phase II studies will focus on further improvement of CEM by incorporating organic and water-soluble hormones and growth facrors into CEM. A serum-free CEM that will optimize the growth of cells in vitro would find wide application in research and biotechnology laboratories. The commercial value of such a product would be significant.