ENZYME CYCLING LIGAND REPLACEMENT ASSAY FOR VIRUSES

这项计划的总体目标是开发敏感的免疫学 用于临床病毒诊断的检测方法。第一阶段活动将是 致力于开发基于化验的微孔化验系统 称为配基替换的原理(一种异质的、竞争性的 化验)，并利用酶循环检测系统实现 需要敏感度。作为模型系统，我们将使用Gengue-2病毒和 抗该菌株的单抗。主要重点将放在 关于几种循环系统的评价--关于生产 病毒抗原与酶或辅酶的偶联物 循环系统，以及对配体替换效果的评估 用于病毒诊断的化验。第一期工程圆满完成 活动将导致第二阶段的实验，以纳入所有试剂 转换成适合小型诊所和医生使用的干胶片格式 办公室。第二阶段的完成将提供高度敏感的病毒 检测系统既可用于设备齐全的临床 实验室以及小诊所和其他“实地”情况。

It is the overall goal of this project to develop sensitive immunological assays for use in clinical viral diagnosis. Phase I activities will be devoted to development of a microwell assay system based on an assay principle known as ligand replacement (a type of heterogeneous, competitive assay) and utilizing enzyme-cycling detection systems to achieve the sensitivity required. As a model system, we will use gengue-2 virus and monoclonal antibodies against this strain. Major emphasis will be placed on the evaluation of several cycling systems, on the production of conjugates of virus antigen with enzymes or coenzymes appropriate to the cycling systems, and on evaluating the efficacy of the ligand replacement assay for use in viral diagnosis. Successful completion of Phase I activities will lead in Phase II to experiments to incorporate all reagents into a dry film format suitable for use in small clinics and physicians' offices. Completion of Phase II will make available highly sensitive virus detection systems which can be used both in well equipped clinical laboratories as well as small clinics and other "field" situations.