RAPID PRESSURE RISE: VASCULAR RESISTANCE AND STEREOLOGY

这是一项研究兔耳变化的时间进程的建议。 部分腹主动脉后的血管阻力和结构 收缩(PAAC)。具体目标包括：(1)确定 耳血管床的体外阻力-流量-压力-特性 从正常血压和高血压兔，(2)定量 已知影响血管阻力的形态参数 直接即不同大小的血管的数量、长度和半径， (3)用定量的体视学术语描述 主动型和被动型动脉壁 不同大小的动脉的特征，(4)大小的测量， 应用ESTABLISHED进行的中膜平滑肌细胞的定位和数量 体视学方法，(5)肌细胞和内皮细胞的定量 与比例组成和组织有关的超微结构 收缩装置、细胞器和膜。这些研究将是 使用各种方法和技术：(1)手术后 收缩主动脉以启动时间进程研究，(2)垂直 耳部血管系统流量-压力曲线的试管测定 支持恒定流量和压力测量，(3)阻力 交感神经激动剂和神经刺激反应的测量(4) 功能定义条件下的压力固定，(5) 微血管造影术和扫描电子显微镜测定 血管几何学，(6)光和组织的塑料包埋 透射电子显微镜，(7)形态测量学和体视学 图像分析图形计算机系统。总的来说，这些研究应该 进一步了解基本泛函和基本泛函之间的相关性 发展中的高血压的结构性变化。具体地说，建议的 这项研究将详细调查一个完整的血管床，以确定 压力和血管阻力的变化与血管形态计量学有关 血管壁的血管网络和体视学。

This is a proposal to study the time course of changes in rabbit ear vascular resistance and structure after partial abdominal aortic constriction (PAAC). The specific aims include: (1) determination of the in vitro resistancae-flow-pressure-characteristics of the ear vascular bed from normotensive and hypertensive rabbits, (2) quantitation of the morphological parameters which are known to effect the vascular resistance directly i.e. the number, length and radius of the different sized vessels, (3) description in quantitative stereological terms of the components in the arterial wall which are responsible for the active and passive characteristics of the various sized arteries, (4) measurement of the size, orientation and number of medial smooth muscle cells using established methods of stereology, (5) quantitation of muscle cell and endothelial ultrastructure with regard to proportional composition and organization of the contractile apparatus, organelles and membranes. These studies will be pursued using a variety of approaches and techniques: (1) post-surgical constriction of the aorta to initiate time course studies, (2) vertical tube determination of flow-pressure curves for the ear vasculature with supporting constant flow and pressure measurements, (3) resistance measurement in response to sympathetic agonists and nerve stimulation, (4) pressure fixation under functionally defined conditions, (5) microangiographic and scanning electron microscopic determination of vascular geometry, (6) plastic embedding of tissues for light and transmission electron microscopy, (7) morphometry and stereology with the image analysis graphics computer system. In general, these studies should further our knowledge of correlation between basic functional and structural changes in developing hypertension. Specifically, the proposed study will investigate one intact vascular bed in detail to determine how changes in pressure and vascular resistance are related to morphometry of the vascular network and stereology of the vascular wall.