BIOCHEMICAL MECHANISM OF ACTION OF VITAMIN A

维生素A已被证明在正常生长，细胞 分化、繁殖和视觉。 很多人都知道 然而，维生素A缺乏症的分子基础 作用机制只有在视觉上才能清楚地理解 周期 随着最近克隆的核维甲酸 受体很可能是维甲酸， 视黄醇可通过核受体与特定基因相互作用 引起转录的调节。 我们建议使用分子克隆技术来鉴定mRNA 维生素A、视黄醇和维生素A 酸在测试中是大鼠的。 一旦这些记录被 确定后，我们计划（1）进行分子表征;（2） 检查他们在测试和其他睾丸外的表达 组织和细胞系;和（3）鉴定类维生素A特异性 在代表性数量的类维生素A的启动子中， 调节基因 这些实验将提供重要的信息， 对最初事件的理解 维生素A营养的病理学和生物化学分支。

Vitamin A has been shown to play a role ln normal growth, cell differentiation, reproduction and vision. Much is known about the pathology of vitamin A deficiency, however, the molecular basis of the mechanism of action Is only clearly understood In the visual cycle. With the recent cloning of the nuclear retinoid acid receptor it is very Likely that retinoid acid and potentially retinol can Interact with specific genes via the nuclear receptors causing modulation of transcription. We propose to use molecular cloning techniques to Identify mRNAs which are directly modulated by vitamin A, retinol and retinolic acid in the test is of the rat. Once these transcripts have been Identified we plan (1) to molecularly characterize then; (2) examine their expression in the tests and other extratesticular tissues and cell Lines; and (3) identify retinoid specific sequences In the promoter of a representative number of retinoid- modulated genes. These experiments will provide important information pertinent to the understanding of the initial events involved In the pathological and biochemical ramifications of vitamin A nutrition.