BIOCHEMICAL MECHANISM OF ACTION OF VITAMIN A

维生素A已被证明在正常生长、细胞 分化、繁殖和视觉。人们对此已有很多了解 然而，维生素A缺乏的病理学，其分子基础 只有在视觉上才能清楚地理解其作用机制。 周而复始。随着最近核维甲酸的克隆 受体很可能是维甲酸和潜在的 视黄醇可以通过核受体与特定基因相互作用 导致转录的调节。 我们建议使用分子克隆技术来识别mRNAs 它们直接受维生素A、视黄醇和视黄醇的调节 测试中的酸是老鼠的。一旦这些文字记录被 我们计划(1)对其进行分子表征；(2) 检查他们在测试和其他特殊情况下的表达 组织和细胞系；以及(3)鉴定维甲酸特异性 具有代表性的维甲酸启动子中的序列- 经过调节的基因。 这些实验将提供有关以下方面的重要信息 对涉及的初始事件的理解 维生素A营养的病理和生化分支。

Vitamin A has been shown to play a role ln normal growth, cell differentiation, reproduction and vision. Much is known about the pathology of vitamin A deficiency, however, the molecular basis of the mechanism of action Is only clearly understood In the visual cycle. With the recent cloning of the nuclear retinoid acid receptor it is very Likely that retinoid acid and potentially retinol can Interact with specific genes via the nuclear receptors causing modulation of transcription. We propose to use molecular cloning techniques to Identify mRNAs which are directly modulated by vitamin A, retinol and retinolic acid in the test is of the rat. Once these transcripts have been Identified we plan (1) to molecularly characterize then; (2) examine their expression in the tests and other extratesticular tissues and cell Lines; and (3) identify retinoid specific sequences In the promoter of a representative number of retinoid- modulated genes. These experiments will provide important information pertinent to the understanding of the initial events involved In the pathological and biochemical ramifications of vitamin A nutrition.