MECHANISMS IN THE REGULATION OF PROLACTIN SECRETION

哺乳动物垂体前叶催乳素的分泌 会受到各种激素和激素的持续修饰 神经递质。反过来，这些调制器通常会演示 多个操作和/或对多个单元格类型的操作。这项研究 对催乳素的调节作用因 催乳素分泌细胞亚群可能表现为 对不同调节剂的反应不同。这个 这些调节剂对催乳素的联合或顺序作用 对于释放，人们知之甚少。此外，研究评估 这些因素的个体影响主要利用了 不考虑脑垂体组织或细胞制剂 乳酸菌之间的功能异质性。因此，最终的 关于推测催乳素的生理作用的结论 监管机构仍然难以捉摸。 为了了解催乳素分泌调节剂是如何工作的 同时，首先有必要定义它们是如何单独进行的。在……里面 此外，催乳素分泌亚群的鉴定 受这些监管机构的不同影响是固有的 理解。这份提案概述了一个多学科的 多巴胺作用机制研究的实验方法(S) 抑制单个乳酸菌中催乳素的释放 从正常大鼠垂体前叶组织中分离出来。可行的 分泌催乳素的细胞将使用 免疫学反向溶血空斑试验。催乳素 秘书将根据1)催乳素的多少进行分组 它们释放(大斑块-与小斑块形成者)，或2) 无论它们是只释放催乳素还是同时释放催乳素和 生长激素。这些细胞将被用来研究细胞 以及使用巨细胞贴片对多巴胺的膜反应 钳夹技术。这项技术的多功能性将使 对细胞内信使系统的作用也进行了评估 作为膜离子机制在多巴胺的调节中 S对催乳素释放的作用。 对潜在的作用机制的表征 单独的调制器将建立必要的基础 了解完整的神经内分泌功能 催乳素分泌的生理变化。此外，这一点 研究将提供一个框架，在其中检查细胞 以及与之相关的膜特性和异常 激素分泌的病理学。因此，这些研究具有 直接关系到生育和生殖的调节以及 在临床治疗高催乳素血症中的作用。

Secretion of prolactin in the mammalian anterior pituitary gland is subject to continual modification by a variety of hormones and neurotransmitters. These modulators, in turn, often demonstrate multiple actions and/or actions on multiple cell types. The study of prolactin regulation is further complicated by the existence of subpopulations of prolactin-secreting cells which may exhibit differences in responsiveness to various modulators. The combined or sequential effects of these modulators on prolactin release are poorly understood. Furthermore, studies evaluating the individual effects of these factors have primarily utilized pituitary tissue or cell preparations without taking into account the functional heterogeneity among lactotrophs. Thus, definitive conclusions regarding the physiological roles of putative prolactin regulators remain elusive. In order to understand how regulators of prolactin secretion work in concert, it is first necessary to define how they individually. In addition, identification of subpopulations of prolactin secretors differentially affected by these regulators is inherent to this understanding. This proposal outlines a multidisciplinary experimental approach to the study of mechanism(s) of dopamine inhibition of prolactin release from individual lactotrophs dissociated from normal rat anterior pituitary tissue. Viable prolactin-secreting cells will be identified using the immunologically-based reverse hemolytic plaque assay. Prolactin secretors will be subgrouped according to 1) how much prolactin they release (large plaque- vs. small plaque-formers), OR 2) whether they release only prolactin or release both prolactin and growth hormone. These cells will then be used to study cellular and membrane responses to dopamine using the gigaseal patch clamp technique. The versatility of this technique will enable evaluation of the roles of intracellular messenger systems as well as membrane ionic mechanisms in the mediation of dopamine's action(s) on prolactin release. Characterization of the mechanisms underlying the action of individual modulators will establish the necessary basis for understanding integrated neuroendocrine function during physiological changes in prolactin secretion. In addition, this research will provide a framework in which to examine cellular and membrane properties and abnormalities associated with pathologies of hormone secretion. As such, these studies have a direct bearing on the regulation of fertility and reproduction and in the clinical management of hyperprolactinemia.