MOLECULAR REGULATION OF NEUTROPHIL ADHERENCE RECEPTOR

求职者：近期职业目标：我的近期职业目标是 继续我的发展作为一个学术血液学家和独立的 调查员 我的重点是基础研究。 我的校长 研究领域涉及中性粒细胞的分子生物学 整合素分子。 应聘者：长期职业目标：我的长期职业目标是 我的大部分时间（80-90%）用于骨髓的基本检查 分化 FIRST奖在发展中的作用作为一个独立的研究者： FIRST Award将为我提供资源和时间来追求我自己的梦想。 我在西雅图退伍军人医疗中心的实验室里进行研究。 机构发展规划与环境：西雅图退伍军人医疗中心 中心和华盛顿大学医学系 医学院都非常支持的发展基础 研究，特别是我的研究计划。 西雅图 VA医疗中心是华盛顿大学的一个组成部分 医疗计划，和西雅图退伍军人医疗中心的一个主要方面是 一个由许多独立调查员组成的医学研究服务机构 由NIH和非NIH基金资助。西雅图退伍军人医疗中心 作出了重大承诺，以了解和处理 血液系统疾病，骨髓移植设施正在运行， 五个研究实验室研究造血的各个方面。 此外，华盛顿大学医学院和 弗雷德哈钦森癌症研究中心为西雅图提供了丰富的 血液学和血液恶性肿瘤研究的多样性。 研究概要：本研究的目的是：1） 确定参与维甲酸诱导的细胞凋亡的调控序列， 中性粒细胞CD 18（β）亚单位基因的转录激活， 分化HL-60早幼粒细胞，和2）鉴定 调节序列参与维甲酸诱导的稳定 CD 11b（Mac-l α）转录物在分化的HL-60细胞中的表达。

Candidate: Immediate Career Goal: my immediate career goal is to continue my development as an academic hematologist and independent investigator. My major focus is that of basic research. My principal area of investigation involves the molecular biology of the neutrophil integrin molecules. Candidate: Long-Term Career Goal: My long-term career goal is to devote the majority o my time (80-90%) to basic investigation of myeloid differentiation. Role of FIRST Award in Developuent As An Independent Investigator: The FIRST Award will provide the resources and time for me to pursue my own research ideas in my own laboratory at the Seattle VA Medical Center. Institutions Development Plans and Enviroment: The Seattle VA Medical Center and the Department of medicine at the University of Washington School of Medicine are very supportive of the development of basic research in general, and my research program in particular. The Seattle VA Medical Center is an integral part of the University of Washington Medical Program, and a major aspect of the Seattle VA Medical Center is a Medical Research Service with a number of independent investigators supported by NIH and non-NIH funds. The Seattle VA Medical Center has made a major commitment to the understanding and treatment of hematologic disorders with an active bone marrow transplant facility and five research laboratories studying various aspects of hematopoiesis. In addition, the University of Washington School of Medicine and the Fred Hutchinson Cancer Research Center provide Seattle with a rich diversity for study of hematology and hematologic malignancy. Brief Sununary of Research: The goals of this research are to: 1) identify the regulatory sequences involved in the retinoic acid-induced transcriptional activation of the neutrophil CD18 (beta) subunit gene in differentiating HL-60 promyelocytic cells, and 2) identify the regulatory sequences involved in the retinoic acid-induced stabilization of the CD11b (Mac-lalpha) transcript in differentiating HL-60 cells.