MOLECULAR POPULATION GENETICS OF ADH IN DROSPHILA

这项研究的目的是分析种内 和种间变异的核苷酸序列 表型单态性位点，以确定重要的 产生和维持核苷酸多样性的进化力量 在自然人群中。 核苷酸多样性是 表型变异的来源，使生物体能够适应 自然种群面临的新的环境挑战， 获得抗病能力。 因此，重要的是要了解 调节基因水平的进化力量 自然种群的变异。 此外，进化 表型简单的遗传位点的动力学必须是 在形成这种模式的进化力量之前， 表型复合体的核苷酸多样性和组织 可以确定位置。 这项拟议中的研究将对100个核苷酸多样性样本进行抽样， 醇脱氢酶（Adh）区的核苷酸序列， 表型单态位点，从五个自然群体的 拟暗果蝇（Drosophila pseudobscura）和一个D. Persimilis to 测试这个轨迹是否符合中性理论的预测 分子进化的一部分。 表型单态性基因座应该 根据中性理论的预测， 缺乏其他进化力量，如自然选择。 任何与中立者预测的偏差 在D. pseudosobscura将表明， 自然选择的作用可能会塑造这种模式， 核苷酸多样性在表型上的组织 单态位点

The objective of the proposed study is to analyze the intraspecific and interspecific variation of nucleotide sequences of a phenotypically monomorphic locus to determine the important evolutionary forces that generate and maintain nucleotide diversity in natural populations. Nucleotide diversity is the ultimate source of phenotypic variation which allows organisms to adapt to new environmental challenges in natural populations such as acquiring disease resistance. Thus, it is important to understand the evolutionary forces that modulate the levels of genetic variation in natural populations. In addition, the evolutionary dynamics of a genetic locus that is phenotypically simple must be understood before the evolutionary forces that shape the pattern and organization of nucleotide diversity of phenotypically complex loci can be ascertained. The proposed study will sample the nucleotide diversity of 100 nucleotide sequences of the alcohol dehydrogenase (Adh) region, a phenotypically monomorphic locus, from five natural populations of Drosophila pseudoobscura and one population of D. persimilis to test whether this locus fits the predictions of the neutral theory of molecular evolution. A phenotypically monomorphic locus should behave according to the predictions of the neutral theory in the absence of other evolutionary forces such as natural selection. Any departures from the predictions of the neutral model seen in the Adh region of D. pseudoobscura will suggest that the action of natural selection may shape the pattern and organization of nucleotide diversity at a phenotypically monomorphic locus.