MECHANISM OF ACTION OF YEAST CDC8 PROTEIN IN DNA REPLICA

这项提议的长期目标是研究分子 真核细胞DNA复制的机制。低等真核生物 生物酵母菌将被用作模型系统。一名活跃分子 酵母DNA复制的成分CDC8被选择用于进一步 学习。 我们已经对CDC8基因进行了分析，并鉴定其基因产物为 酵母胸苷酸激酶。我们将进一步研究亚细胞 CDC8蛋白的定位及其功能研究 通过细胞分裂周期。CDC8之间的关系 本课程将探讨蛋白质和其他复制成分。这个 结果将与其他真核生物的功能进行比较 复制系统。这项研究将提供有关 酵母复制组件的时间和空间事件以及 CDC8在DNA复制中的作用机制。遗传 对CDC8作用的探讨将进一步证实上述观点 学习。 CDC8抑制物(SOC8-1)也将被研究。该基因及其相关基因 基因产品将被表征，结果将是 整合到CDC8研究的信息中，为我们提供 推测CDC8抑制机制和作用的信息 在DNA复制中。 我们相信，这项研究将有助于我们理解 DNA前体酶的代谢协调和 参与传播和调节的复杂过程 酵母DNA复制。我们期待着酵母的澄清 酶将导致对相应的功能的理解 其他真核系统中的酶。

The long-term goal of this proposal is to investigate the molecular mechanism of eukaryotic DNA replication. The lower eukaryotic organism, yeast, will be used as the model system. An active component of yeast DNA replication, CDC8, is selected for further study. We have analyzed the CDC8 gene and identified its gene product as yeast thymidylate kinase. We will further examine the subcellular localization of the CDC8 protein and investigate its functions through the cell division cycle. The relationship between the CDC8 protein and other replication components will be explored. The result will be compared to the functions of other eukaryotic replication systems. This study will provide information about the temporal and spatial events of yeast replication components and the mechanism of action of the CDC8 in DNA replication. Genetic approach to the roles of CDC8 will further substantiate the above studies. A CDC8 suppressor (SOC8-1) will also be studied. The gene and its gene product will be characterized, and the result will be integrated into information from the CDC8 studies, providing us information to deduce the suppression mechanism and role of CDC8 in DNA replication. We believe that this research will contribute to our understanding of the metabolic coordination of DNA precursor enzymes and the complex processes involved in the propagation and regulation of yeast DNA replication. We expect that the elucidation of yeast enzymes will lead to understanding the functions of corresponding enzymes in other eukaryotic systems.