REGENERATIVE POTENTIAL OF MUSCLE SPINDLES AFTER TRAUMA

我们计划研究哺乳动物肌梭的潜力， 骨骼肌损伤后的恢复。 自体移植的 （移植的）大鼠比目鱼肌被用作实验模型， 创伤后肌肉再生和修复。 我们的目标是探索 移植肌肉中肌梭的起源和形成，以及 建立再生纺锤体的结构和神经支配模式。 一个重要的目标是确定纺锤体是否会重新形成。 到 回答这个问题，我们将移植二腹肌，肌肉缺乏 纺锤体，进入以前由含纺锤体的肌肉占据的部位（a 具有形成纺锤体的潜力的位点）。 另一个目的是 评估感觉和运动神经纤维在 确定再生纺锤体的结构。 为此我们 将检查在缺乏的情况下再生的肌肉中的纺锤体。 感觉或运动神经支配。 运动轴突的神经营养作用 梭内肌纤维的性质将与 通过比较在一个部位再生的肌梭的活动， 缺乏运动神经支配和那些在静止状态下再生的， 运动神经支配的肌肉到正常的大鼠纺锤体。 最后，我们将 确定再生肌肉中的纺锤体是否具有形态学特征， 以及运动和感觉神经支配 让他们以正常的方式运作。 本研究将采用连续切片的组织学和组织化学方法 技术，采用光学和电子显微镜。 冷冻组织 将对描绘梭内肌类型的酶进行染色 纤维，以及感觉和运动神经支配。 嵌在树脂里的肌肉 将在连续切片中染色，以重建分支 感觉和运动轴突进入纺锤体的模式和检查 神经末梢的形式。 这些技术允许详细 本文对正常和非正常的纺锤体结构进行了定量比较， 再生肌肉 再生肌移植中肌梭的观察 有助于理解移植或受伤的肌肉是如何 重新设立为职能实体。

We plan to examine the potential of mammalian muscle spindles for restoration following skeletal muscle injury. The autografted (transplanted) rat soleus muscle is used as an experimental model of post-traumatic muscle regeneration and repair. Our aim is to explore the origin and formation of muscle spindles in the grafted muscles, and to establish the structure and patterns of innervation of regenerated spindles. An important goal is to determine whether spindles will form anew. To answer this question, we shall graft the digastric, a muscle devoid of spindles, into a site formerly occupied by a spindle-containing muscle (a site with potential for the formation of spindles). Another objective is to assess the respective roles of sensory and motor nerve fibers in determining the structure of regenerated spindles. For this reason, we shall examine spindles in muscles that have regenerated in the absence of sensory or motor innervation. Neurotrophic influences of motor axons on properties of intrafusal fibers will be separated from influences of activity by comparison of muscle spindles that have regenerated in a site devoid of motor innervation and those that have regenerated in quiescent, motor-innervated muscles to normal rat spindles. Lastly, we shall determine whether spindles in the regenerated muscle have morphological properties and motor and sensory innervation of the kind that would allow them to function in a normal manner. The study will use serial-sectioning histological and histochemical techniques, employing both light and electron microscope. Frozen tissues will be stained for enzymes which delineate types of intrafusal muscle fiber, as well as sensory and motor innervation. Muscles embedded in resin will be stained in serial sections to allow reconstructions of branching patterns of sensory and motor axons entering the spindles and examination of the form of nerve terminals. These techniques permit detailed quantitative comparison of the structure of spindles in normal and regenerated muscles. Investigation of muscle spindles in regenerating muscle grafts will contribute to the understanding of how transplanted or injured muscles are re-established as functional entities.