REGULATORY MECHANISMS IN THE IMMUNE RESPONSE

这项建议的目的是详细了解 T细胞帮助B细胞对抗原做出反应。我们是 对发生的两个过程特别感兴趣；第一， 启动B细胞反应的事件，其次，对 淋巴因子B细胞刺激因子-1(BSF1)。 尽管淋巴因子本身可以驱动B细胞对 B细胞对多种抗原的某些抗原反应，尤其是 那些出生在蛋白质上的人，需要携带者特异性T细胞的存在 细胞和淋巴因子。这些T细胞似乎参与了 主要是在B细胞反应的开始阶段。他们可能是 通过分泌一些迄今未被发现的淋巴因子或通过 它们自己的膜蛋白与T上的配体直接接触 或B细胞和/或分离的膜蛋白，我们计划 调查以下哪种可能性是正确的。膜 要研究的成分包括T细胞受体，L3T4， LFA-1和II类分子。例如，我们将隔离 来自特定辅助T细胞杂交瘤的受体并通过 这些受体是否与抗原+结合的各种策略 MHC。这些受体将用于脂质体或在此之后 转移到另一个T细胞杂交瘤进行抗原特异性检测 帮助者活动。 BSF1对B细胞有多种影响。它诱导Ia合成 在静息细胞中，并刺激亚最大增殖 激活的B细胞。我们计划分离B细胞上BSF1的受体 细胞，并详细研究这种淋巴因子的诱导作用。 没有休眠细胞。

The aims of this proposal are to understand in detail the means whereby T cells help B cells respond to antigen. We are particularly interested in two processes which occur; first, the events which initiate B cell response and secondly, the effects on B cells of the lymphokine B cell stimulating factor-1 (BSF1). Although lymphokines alone can drive the responses of B cells to some antigens responses of B cells to many antigens, particularly those born on proteins, require the presence of carrier-specific T cells as well as lymphokines. These T cells appear to be involved chiefly at the beginning of the B cell response. they may be acting by secreting some hitherto undiscovered lymphokine or by direct contact of their own membrane proteins with ligands on T or B cells and/or isolated membrane proteins, we plan to investigate which of these possibilities is correct. Membrane components to be studied will include the T cell receptor, L3T4, LFA-1 and Class II molecules. For example, we will isolate receptors from a particular helper T cell hybridoma and test by various strategies whether these receptors bind antigen plus MHC. These receptors will be used in liposomes or after transfer to another T cell hybridoma to test for antigen-specific helper activity. BSF1 has a number of effects on B cells. It induces Ia synthesis in resting cells, and stimulates proliferation of sub-maximally activated B cells. We plan to isolate the receptor for BSF1 on B cells, and study in detail the inductive effects of this lymphokine no resting cells.