IMMUNOASSAY FOR HUMAN PROGESTERONE RECEPTOR

孕激素受体是一类类固醇激素 已被证明具有重要调节作用的受体 对乳腺和其他靶组织的影响。质的和 黄体酮受体的定量测定在以下方面很有用， 例如，乳腺癌的治疗管理。 目前，现有的孕激素受体检测方法有 使其难以在大多数临床上使用的缺点 实验室。这项提议的主要长期目标是 一种灵敏可靠的酶联免疫分析方法的建立 对于孕激素受体，产生的单抗 能够区分不同形式的 受体及其纯化的人孕酮的分离 使用免疫亲和树脂的受体，所述免疫亲和树脂由以下一种制备而成 上述单抗。

Progesterone receptor is one of a group of steroid hormone receptors that has been shown to have important regulatory effects in mammary and other target tissues. The qualitative and quantitative determination of progesterone receptor is useful in, for example, the therapeutic management of breast cancer. Currently, available assays for progesterone receptor have drawbacks that make them difficult to use in most clinical laboratories. The major longterm goals of this proposal are the development of a sensitive, reliable enzyme linked immunoassay for progesterone receptor, the production of monoclonal antibodies capable of distinguishing between the various forms of the receptor, and the isolation of purified human progesterone receptor using an immunoaffinity resin prepared by using one of the above monoclonal antibodies.