PRODUCTION OF RECOMBINANT PROTEINS IN MILK

一种在牛奶中生产重组蛋白的方法将是 发展起来的。第一阶段将使用一个使用老鼠的模型系统。 这将证明有可能在体内产生新的蛋白质 乳房组织中的牛奶。一种乳腺特异性启动子 细胞将被用来指导一种测试蛋白的表达 很容易被检测到。含有这些的DNA结构 这些片段将被注入小鼠胚胎中。由此产生的 转基因小鼠将接受新病毒的检测 他们的牛奶中含有重组蛋白质。 那些在鼠标模型系统中起作用的构造将 在第二阶段注入牛胚胎。由此产生的 转基因牛将被测试用于生产 他们的牛奶中含有重组蛋白质。这一程序将确立 一种生产新型克隆蛋白质的方法学 以商业基础上的牛奶，成本只有 目前的组织培养技术。

A method for production of recombinant proteins in milk will be developed. A model system using mice will be used in Phase I. This will establish that it is possible to produce novel proteins in milk from mammary tissues. A promoter specific for mammary cells will be used to direct the expression of a test protein that is easily detected. The DNA constructs containing these segments will be injected into mouse embryos. The resulting transgenic mice will be tested for the presence of the new recombinant proteins in their milk. Those constructs that function in the mouse model system will be injected into bovine embryos in Phase II. The resulting transgenic cattle will be tested for the production of recombinant proteins in their milk. This procedure will establish a methodology by which novel cloned proteins can be produced in cow milk on a commercial basis at a fraction of the cost of current tissue culture techniques.