ELECTROFUSION AND ELECTROPERMEATION OF CELLS

利用电穿孔技术穿透细胞 膜以便引入药物或外来遗传物质 转化成活细胞 电融合是一种新的方法， 用于遗传学研究的杂交细胞和单克隆抗体 生产，以及用于通过脂质体的药物输注。 我们计划 开发电穿孔和电融合的方案， 常见的细胞类型，基于我们对物理的理解， 细胞的特性。 电子电池定影器和 将根据我们的方案设计穿孔器。 显微镜下， 宏观和连续流动的细胞融合室将是 进行了 两种专有硬件的成功开发 和软件（程序和公式）将构成基础， 商业化 布法罗生物医学社区提供 为生物技术转让创造良好的环境， 反馈，以及未来扩展的测试市场。

The technique of electroporation is utilized to permeate cell membranes so as to introduce drugs or foreign genetic materials into living cells. Electrofusion is used as a new approach to form hybrid cells for genetics studies and monoclonal antibody production, as well as for drug infusion via liposomes. We plan to develop protocols for electroporation and electrofusion of common cell types, based on our understanding of the physical properties of cells. Prototypes of electronic cell fuser and porator will be designed according to our protocols. Microscopic, macroscopic and continuous-flow cell fusion chambers will be made. The successful development of both proprietary hardwares and softwares (procedures and formulae) will form a basis for commercialization . The Buffalo biomedical community provides an excellent environment for biotechnology transfer and feedback, as well as a test market for future expansion.