SURGICAL STUDIES ON METABOLISM OF G I HORMONES

这项提案的目标是调查 消化(及相关)过程中的胃肠激素(GIH)。 我们将研究神经降压素(NT)从肠道释放的机制 以及甲状腺髓样癌。我们将确定其效果 对不同途径刺激的胃液分泌的影响。我们计划 胆囊素(CCK)、NT、蛙皮素和YY(PYY)多肽的表征 高效液相色谱柱。我们将确定不同的肝脏摄取不同 内源性释放的CCK的生物测定和分子形式 放射免疫分析。我们计划研究CCK的作用机制。 胆囊壁(GB)平滑肌。我们将研究PYY对胃的作用 胃窦和胃底PYY受体的分泌和测定。我们计划研究一下 CCK和TO对Oddi括约肌松弛的作用机制 通过受体研究，确定P物质是否参与其中。我们会 确定肠道和胰腺腺泡之间的关系，并注意 特别是NT、前列腺素和消炎痛的作用。我们会 使用完整的动物、分离的胰腺和分散的腺泡。我们计划 妊高征肠胰岛素与钙调节关系的研究 多肽对胰岛素和胰升糖素的分泌，以确定是否 侧脑室注射胃肠道激素与钙调节 多肽影响胰岛素和胰升糖素的分泌，并检测 妊娠对胃肠激素与性激素关系的影响 胰腺内分泌激素分泌。我们将研究GIH在 对胰腺生长的抑制和促进作用及其机制 与激素受体的关系及作用效果。我们会研究 刺激性和抑制性多肽在糖尿病发病机制中的作用 实验性急性胰腺炎的病程。我们计划确定 促性腺激素释放激素对小鼠肿瘤生长的刺激和抑制作用 肠道和胰腺。我们将测量肠道和胰腺中的GIH受体 癌细胞。我们计划对妊娠高血压综合征和妊娠高血压综合征的关系进行临床研究。 慢性胰腺炎患者手术前后的变化。我们会 继续我们对佐林格-埃里森患者的代谢研究 综合征(ZES)与全胃切除后营养的比较 并不影响患者的健康。我们将研究GIH在慢性阻塞性肺疾病患者中的作用 类甲状腺疾病。我们将研究NT在人体内的代谢。我们会 确定怀孕不同阶段的影响，并与 产后状态下CCK与GB收缩的关系 女人。

The objectives of this proposal are to investigate the role of gastrointestinal hormones (GIH) in the digestive (and related) processes. We will study the mechanisms for release of neurotensin (NT) from the gut and from medullary carcinoma of the thyroid. We will determine the effect of NT on gastric secretion stimulated by different routes. We plan to characterize cholecystokinin (CCK), NT, bombesin, and peptide YY (PYY) by HPLC. We will determine the differential hepatic uptake of different molecular forms of endogenously released CCK by bioassay and radioimmunoassay. We plan to study the mechanisms of actions of CCK on gallbladder (GB) smooth muscle. We will study the action of PYY on gastric secretion and measure PYY receptors in antrum and fundus. We plan to study the mechanism of relaxation of the sphincter of Oddi by CCK and to determine, by receptor studies, whether substance P is involved. We will determine the relationships between the gut and pancreatic acinus and note particularly the roles of NT, prostaglandins, and indomethacin. We will use whole animals, isolated pancreas, and dispersed acini. We plan to characterize the enterinsulinar relationships of GIH and calcium-regulatory peptides on secretion of insulin and glucagon, to determine whether intracerebroventricular administration of GI and calcium-regulatory peptides influence secretion of insulin and glucagon, and to examine the influence of pregnancy on the relationship between gut hormones and pancreatic endocrine hormone secretion. We will study the role of GIH in the inhibition and stimulation of growth of the pancreas and the relationship with hormone receptors and efficacy of action. We will study the role of stimulatory and inhibitory peptides in the pathogenesis and course of experimental acute pancreatitis. We plan to determine the effects of GIH on the stimulation and inhibition of growth of cancers of the gut and pancreas. We will measure GIH receptors in gut and pancreatic cancer cells. We plan clinical studies on the relationship between GIH and chronic pancreatitis in patients before and after operation. We will continue our metabolic studies on patients with the Zollinger-Ellison syndrome (ZES) and plan to compare nutrition after total gastrectomy in ZES and non-ZES patients. We will study the role of GIH in patients with thryoid disease. We will study the metabolism of NT in man. We will determine the effects of various stages of pregnancy, as compared with the postpartum state, on the relationship between CCK and GB contraction in women.