DEVELOPMENT OF ANTI-IDIOTYPE ANTIBODY VACCINES FOR AIDS

该提案的长期目标是开发疫苗 用于艾滋病的抗独特型抗体。 实现 为实现这一目标，第一阶段提出了以下任务：1） 用抗HIV蛋白单克隆抗体（MAb）免疫小鼠， 能够在体外中和病毒生长， 产生抗独特型抗体的杂交克隆，2） 用抗独特型单克隆抗体免疫动物， 用于产生针对HIV蛋白的免疫应答，和 3)以确定是否血清（抗抗独特型抗体）， 免疫动物能够中和体内HIV的生长， 体外培养系统 这些结果将确定利用抗- 独特型抗体引起中和反应， 艾滋病毒，并将提供一个基础的发展， 有效的艾滋病疫苗

The long range objective of the proposal is to develop a vaccine for AIDS using as a basis anti-idiotypic antibodies. To achieve this goal the following tasks are proposed in Phase I: 1) to immunize mice with anti-HIV protein monoclonal antibody (MAb) which are capable of neutralizing viral growth in vitro and to select for hybrid clones which are producing anti-idiotypic antibody, 2) to immunize animals with the anti-idiotypic MAb and subsequently test for the generation of an immune response against HIV proteins, and 3) to determine if sera (anti-anti-idiotypic antibody) from immunized animals are capable of neutralizing HIV growth in an in vitro system. These results will determine the feasibility of utilizing anti- idiotypic antibodies to illicit a neutralizing response against HIV, and additionally will provide a basis for the development of an efficacious vaccine against AIDS.