LIPOSOMES FOR DELIVERY OF DRUGS TO HAIR-FOLLICLE CELLS

描述：（改编自申请人摘要）。 有必要 刺激毛发生长，用于各种脱发症。 断言 为了实现毛发生长刺激， 必须在毛囊细胞内传递， 鉴定活性化合物及其递送方法是使用一种 体外培养系统 原则上，活性化合物也可用于引起 改变头发的颜色和外观。 陈述到 申请人已经开发了凝胶支撑的三维组织培养 系统（霍夫曼，R.M.，Cancer Cells 3，86-92，1991），其允许完整的 生长和测试皮肤的所有成分，包括角质形成细胞， 真皮成纤维细胞和毛囊细胞，持续10天或更长时间 (Li，L.，例如，Proc. Natl. Acad.Sci.- USA. 88，1908-1912，1991）。 头发 其本身可以从这样的皮肤培养物中生长（Li，L.，例如，手稿 制备）。 脂质体（具有双层结构的球形磷脂囊泡 膜结构）被认为是一种方便的手段， 不同物质从低分子量 从药物到DNA质粒 在此提出，利用组织培养系统 和包含在脂质体中的荧光标记化合物， 研究脂质体与毛囊细胞的相互作用， 用于细胞结合的组合物，以包含在给定的脂质体中。 组成不同的低分子量和高分子量， 水溶性和水不溶性化合物，并找到最佳的 脂质体内容物释放到毛囊中的条件 细胞 在第二阶段，将进行模型化合物的体外研究。 进行，以确定其对人类皮肤培养的影响， 开发用于物质的卵泡内递送的商业系统 影响头发生长、颜色和外观。

DESCRIPTION: (Adapted from the Applicant's Abstract). There is a need to stimulate hair growth for various conditions of alopecia. It is asserted that to achieve hair growth stimulation, biologically active compounds must be delivered inside hair follicle cells and, that the optimal way to identify active compounds and methods of their delivery is to use an in vitro system. Active compounds in principle could be also used to cause desired changes in hair color and appearance. It is stated that the applicants have developed a gel-supported three-dimensional histoculture system (Hoffman, R.M., Cancer Cells3, 86-92, 1991) that allows the intact growth and testing of all components of skin, including keratinocytes, dermal fibroblasts and hair-folliclecells, for a period of 10 days or more (Li, L., et al., Proc. Natl. Acad.Sci.-USA. 88, 1908-1912, 1991). Hair itself can grow from such skin cultures(Li, L., et al., manuscript in preparation). Liposomes (spherical phospholipid vesicles with bilayer membrane structure) are considered a convenient means for the intracellular delivery of different substances from low-molecular-weight drugs to DNA plasmids. It is proposed here, using the histoculture system and fluorescent-labeled compounds included into liposomes, to study liposome interactions with hair-follicle cells, to find the optimal compositions for cell binding, to include into liposomes of a given composition different low-molecular-weight and high-molecular-weight, water-soluble and water-insoluble compounds and to find the optimal conditions for the release of the liposomal content into hair-follicle cells. In Phase II, in vitrostudies with model compounds will be performed to determine their effects on human skin culture in order to develop a commercial system for intra-follicle delivery of substances influencing hair growth, color and appearance.