IMMOBILIZED OXIDO-REDUCTASE ENZYMES IN ORGANIC SYNTHESIS

需要新的试剂用于制备光学纯的 大药厂为此，我们建议评估和制定一项 一种新的有机合成双相生物催化体系。总 目的是开发一种方法，其中辅因子依赖 酶可用于有机溶剂中， 生产高价值化合物，如药物。具体 第一阶段的目标是证明马肝酒精 脱氢酶（HLADH），沿着其辅因子烟酰胺腺嘌呤 二核苷酸（NADH），可以固定在水凝胶中并在水凝胶中操作。 二乙醚以从光学纯化合物制备光学纯化合物 无活性前体。第二阶段将开发这种新的合成 方法学转化为低成本生产的一般程序， 使用辅因子依赖性的药物和农业化合物 有机溶剂中的氧化还原酶。这三位研究人员 提交本提案的合作伙伴成功地开发了 用于立体选择性固定化的不依赖于辅因子的酶系统 在有机溶剂中水解和酯交换。

There is a need for new reagents for the production of optically pure pharmaceuticals. For this purpose, we propose to evaluate and develop a new biphasic biocatalyst system for organic synthesis. The general objective is to develop a methodology in which cofactor dependent enzymes can be used in organic solvents for the stereospecific production of high value compounds such as pharmaceuticals. The specific Phase I objective is to demonstrate that horse liver alcohol dehydrogenase (HLADH), along with its cofactor nicotinamide adenine dinucleotide (NADH), can be immobilized in a hydrogel and operate in diethyl ether to produce optically pure compounds from optically inactive precursors. Phase II will develop this new synthetic methodology into general procedures for the low cost production of pharmaceutical and agricultural compounds using cofactor dependent oxido-reductase enzymes in organic solvents. The three researchers submitting this proposal have collaborated successfully to develop an immobilized, cofactor independent, enzyme system for stereoselective hydrolysis and transesterification in organic solvents.