HIGH-RESOLUTION MUTAGENESIS

探索高分辨率诱变的可行性 L5178Y小鼠淋巴瘤细胞中胸苷激酶(TK)基因座 被检验(阿尔法)以更彻底地评估假说 L5178Y双峰分布中的小西格玛菌落 细胞突变是更广泛的细胞损伤的结果 TK基因的区域(染色体11b)和那个大的(Lambda) 克隆产生于TK基因点突变，和(B) L5178Y小鼠omega和lambda突变的特征 缺叶条件下生长的3.7.2C、LY-S1和LY-R83菌株 介质，然后暴露于乙基甲烷磺酸盐。表型 TK和半乳糖激酶(GK)基因座的突变培养物的数量将是 鉴定；高分辨率显带染色体(第11号) 将被分析，并原位杂交的放射性探针 TK和GK基因的高分辨显带染色体将 被启动。在第二阶段，这些办法将继续下去， 这些突变体将在分子水平上进行表征。

The feasibility of probing high resolution mutagenesis at the thymidine kinase (TK) locus in L5178Y mouse lymphoma cells will be examined (alpha) to more thoroughly evaluate the hypothesis that small sigma colonies in the bimodal distribution of L5178Y cells mutants are the result of more extensive damage to the region of the TK genes (chromosome 11b) and that large (lambda) colonies result from point mutations to the TK gene, and (b) to characterize omega and lambda mutants arising in L5178Y mouse strains 3.7.2C, LY-S1, and LY-R83 grown in folic acid-deficient medium then exposed to ethylmethane sulfonate. The phenotype of mutant cultures at the TK and galactokinase (GK) loci will be characterized; high resolution banded chromosomes (number 11) will be analyzed, and in situ hybridization of radioactive probes of TK and GK cDNA to the high-resolution banded chromosomes will be initiated. In Phase II these approaches will be continued and the mutants will be characterized at the molecular level.