PURIFICATION OF MARINE OIL-DERIVED POLYUNSATURATES

拟议项目的具体目标是开发一种技术上的 一种高效、经济的分离提纯方法 多克量的二十碳五烯酸(EPA，C20：5 omega3) 海洋/鱼油的长期目标是制造这种油，并可能 其他可供等待市场使用的高度多不饱和化合物。这个 拟议项目与健康有关的原因是 花生四烯酸，EPA是前列腺素和 其他具有广泛生理学和病理生理学意义的二十烷类化合物 生物学和医学，以及越来越多的证据表明，当地人(爱斯基摩人， 沿海居民)和富含EPA的实验性饮食 抗血栓/心脏保护作用。该方法适用于 EPA等海洋石油衍生产品的分离提纯 多不饱和化合物将专注于最先进的创新(系统和 分离科学)在制备性高效液相色谱中。 商业应用、商机和潜在市场将反映 广泛而多产的研究活动和兴趣 生化/化学，培养，动物，临床，营养 研究等)世界范围内的前列腺素前体多不饱和 脂肪酸在工业、大学、政府卫生机构、 和研究机构。

The specific aim of the proposed Project is to develop a technologically efficient and cost-effective method for the separation and purification of multi-gram quantities of eicosapentaenoic acid (EPA, C20:5omega3) from marine/fish oils with the long-term objective of making this and possibly other highly polyunsaturates available for a waiting market. The health-relatedness of the proposed Project is due to the fact that, like arachidonic acid, EPA is a biosynthetic precursor of prostaglandins and other eicosanoids of expanding physiologic and pathophysiologic interest in biology and medicine, and accumulating evidence that native (Eskimos, coastal dwellers) and experimental diets rich in EPA have antithrombotic/cardioprotective effects. The methodology for the separation and purification of EPA and other marine oil-derived polyunsaturates would focus on state-of-the-art innovations (systems and separation science) in preparative high performance liquid chromatography. Commercial applications, opportunities, and potential market would reflect the extensive and prolific research activity and interest (biochemical/chemical, in culture, in animal, clinical, nutritional studies, etc.) world-wide in the prostaglandin-precursor polyunsaturated fatty acid cascades in industry, universities, government health agencies, and research institutes.