CHOLESTEROL THRESHOLD ASSAY

建议开发一种用于检测胆固醇的血液试纸。 血浆通过专利过滤从细胞中分离出来 通过玻璃纤维羊毛，收集血浆在一个 可拆卸的接受部件，并将其洗脱成液体试剂。 胆固醇酯酶(CHE)和氧化物酶(CHOD)结合在干粉中 条子上的表格。定量是基于一种“阈值”分析 过氧化氢，由CGE和CHOD产生。化验中含有一种“清道夫”。 和一种“信号”反应。过氧化物量低于临界值， 相当于世界卫生组织最近提出的200毫克/分升胆固醇下限 NHLBI，是在清道夫中反应的，以上临界量在 信号反应。因此，通过消除大的彩色背景，以及 选择高度敏感的信号反应，视觉解释是 大大增强了。该设备不需要制造它的仪器 经济、快速，适用于大规模筛查和自我监测。可行性 已经证明了过氧化氢和胆固醇的测试原理 从血清中提取。 拟议的工作包括最终选择最佳的等离子体分离 和受体成员材料，试剂系统的优化，以及 功能样机试纸的研制 区分10到20 mg/dL左右的浓度区间 200 mg/dL的临界值。

Proposed is the development of a blood test strip for cholesterol. Plasma is separated on the strip from cells by a patented filtration process through a glass fiber fleece, collecting the plasma on a detachable recipient member, and eluting it into a liquid reagent. Cholesterol esterase (CHE) and oxidase (CHOD) are incorporated in dry form on the strip. Quantitation is based on a "threshold" assay for peroxide, generated by CGE and CHOD. The assay comprises a "scavenger" and a "signal" reaction. The below threshold amount of peroxide, equivalent to the 200 mg/dL cholesterol cutoff recently suggested by the NHLBI, is reacted in the scavenger, the above threshold amount in the signal reaction. Thus, by elimination of large color backgrounds, and choosing highly sensitive signal reactions, visual interpretation is greatly enhanced. The device does not require an instrument making it economical and fast for mass screenings and self-monitoring. Feasibility of the test principle has been demonstrated for peroxide and cholesterol from serum. The proposed work comprises final selection of optimal plasma separation and recipient member materials, optimization of the reagent system, and the manufacture of functional prototype test strips capable of differentiating concentration intervals of between 10 and 20 mg/dL around the 200 mg/dL cutoff level.