PRECAST GELS--EXPERIMENTAL ADVANCE IN DNA SEQUENCING

同时鉴定脱氧核糖核酸的核苷酸序列 (DNA)对于分析和理解基因是至关重要的 信息，这项承诺的可行性受到 目前可用的实验进展。重要的是 对人类基因组的解码是众所周知的，并且超出了这一范围 求婚。DNA中核苷酸序列的测定是一种 考虑到发现的大量核苷酸单位，这项任务非同寻常 即使是最小的DNA分子。一种高度可重复性的，容易复制的 可用于核苷酸碱基电泳法分离的载体 DNA对于在这一领域取得重大进展是必不可少的 研究的成果。市售一次测序用预制凝胶 被认为是一种奢侈品，已经成为研究人员的必需品。 这项提议的目标是；开发一种预制的DNA测序凝胶， 一种浇注凝胶并进行凝胶测试的方法 大学研究实验室。该项目的第二阶段将涉及质量问题 测试和解决生产扩大的问题 最终目标是提供高质量和经济的商业 可用的凝胶与目前可用的DNA测序仪兼容。

While identification of the nucleotide sequence of deoxyribonucleic acid (DNA) is critical for the analysis and understanding of genetic information, the feasibility of this undertaking is limited by the experimental advances that are currently available. The importance of decoding the human genome are well known and exceed the scope of this proposal. Determination of the nucleotide sequence in DNA is an extraordinary task considering the large number of nucleotide units found in even the smallest DNA molecule. A highly reproducible, readily available vehicle for electrophoretic separation of the nucleotide bases in DNA is essential in order to achieve a significant advancement in this area of research. Commercially available precast gels for sequencing once considered a luxury, have become a necessity for the researcher. The objective of this proposal is to; develop a precast DNA sequencing gel, a process for pouring the gels and to perform testing of the gels in a university research lab. Phase II of this project will involve quality testing and addressing the problems of production scale-up with the ultimate goal of supplying a high quality and economical commercially available gel compatible with currently available DNA sequencers.