A NOVEL COLORIMETRIC ENZYME ASSAY FOR FIBRINOLYSIS

我们最近已经开发了一种用于该组合物的组分的测定系统。 纤溶途径这种新的分析是基于固相 酶连接的纤维蛋白基质，其中纤溶酶通过释放 从基质中提取酶。我们建议改进某些固相 在方便性方面的分析，并扩展这一点， 一种制备系列活化剂功能性免疫测定的技术 以及活化剂复合物的免疫测定 和该系统的抑制剂。感兴趣的因素包括 激活剂t-PA和尿激酶，纤溶酶抑制剂，α 2- 抗纤溶酶和激活剂抑制剂派-1（纤溶酶原激活剂 抑制剂-1）。我们将研究的复合物是t-PA -派-1， 指示在激活水平的抑制程度，和 α 2 -抗纤溶酶-表明激活程度的纤溶酶 纤维蛋白溶解系统。我们已经安排进行临床 使用来自三个患者群体的血浆的我们的测定的应用。 第一组是冠状动脉旁路移植术患者，他们代表了一组 严重扰动止血，第二组包括一个井 具有低水平传统风险因素的特征性患者 但事实上确实患有冠心病，最后， 一组被诊断为不稳定型心绞痛的患者，一组可能 称为“心肌梗塞前”。这些患者组 应该可以让我们确定的预测和临床实用性， 我们的分析，并提供有价值的信息，激活状态 或抑制纤溶系统在这三个领域， 关心我们还建议使用我们的系统作为基础研究工具， 确定原代细胞产生的纤溶酶原激活剂的类型 兔上皮细胞的培养物。

We have recently developed an assay system for the components of the fibrinolytic pathway. This novel assays sis based on a solid phase enzyme linked fibrin matrix in which plasmin is measured by the release of enzyme from the matrix. We propose to improve certain solid phase facets of the assay in terms of convenience and to extend this technology to produce a series of functional imunoassays for activators and inhibitors: as well as immunoassays for complexes of the activators and inhibitors of this system. The factors of interest include the activators t-PA and urokinase, the plasmin inhibitor, alpha2 - antiplasmin and the activator inhibitor, PAI-1 (plasminogen activator inhibitor -1). The complexes we will study are t-PA -PAI-1 which is indicative of the degree of inhibition at the activation level, and alpha 2 -antiplasmin - plasmin which indicates the extent of activation of the fibrinolytic system. We have arranged to carry out clinical applications of our assay using plasma from three patient populations. The first group is coronary bypass patients who represent a group with serious perturbations of hemostasis, the second group consists of a well characterized patients who have low levels of traditional risk factors for coronary artery disease (CAD) but do in fact have CAD, and finally a group of patients diagnosed as having unstable angina, a group who may be designated "pre myocardial infarction". These groups of patients should allow us to determine the predictive and clinical usefulness of our assays and provide valuable information on the state of activation or inhibition of the fibrinolytic system in these three areas of concern. We also propose use our system as a basic research tool to determine the type of plasminogen activators produced by primary cell cultures of rabbit epithelial cells.