ULTRA-THIN DIAMOND WIRE SAW FOR HISTOLOGY

该项目的具体目标是制造、开发和评估 一种超薄人造金刚石线锯， 试样 6-12钼、钨或不锈钢直径（μ）m 将通过等离子体辅助化学气相沉积对金属丝进行金刚石涂层 （PACVD）以产生磨料涂层。 亚微米大小的钻石 颗粒将以受控的方式以规则的间隔沉积在 电线 现有的钢丝锯机将被用于切割各种 组织样本 目标是实现1（mu）m-50（mu）m厚的切片， 新鲜组织、难以切片的骨、牙齿的光学显微镜评价 或软骨组织，各种硬度和大直径的组织 有机体样本的质量高于目前可用的 技术. 该技术在医疗应用中的实用性 在免疫细胞化学、酶组织化学、诊断 活检标本，药物的影响是预期的。 长期 目标是将组织学线锯机商业化， 现有切片机的替代或辅助技术。 这台机器 将克服传统切片机的局限性， 应用，也将使更精细，更分子化的 各种组织的分析。

The specific aim of this project is to manufacture, develop and evaluate an ultra-thin synthetic diamond wire saw for sectioning biological specimen. 6-12 (mu)m in diameter molybdenum, tungsten or stainless steel wires will be diamond coated by Plasma Aided Chemical Vapor Deposition (PACVD) to create the abrasive coating. The submicron sized diamond grains will be deposited in a controlled fashion at regular intervals on the wire. An existing wire saw machine will be used to section various tissue samples. The goal is to achieve 1(mu)m-50(mu)m thick sections for light microscopic evaluation of fresh tissue, hard to section bone, tooth or cartilage tissue, tissue of various hardnesses and large diameter organ of organism samples of higher quality than is available by current techniques. The usefulness of the technology for medical applications in such fields as immunocytochemistry, enzyme histochemistry, diagnosis of biopsy specimens, and effects of drugs is anticipated. The long term goal is to commercialize a histological wire saw machine as an alternative or ancillary technology to existing microtomes. This machine will overcome the limitations of conventional microtomes in a number of applications and will also make possible a finer and more molecular analysis of various tissues.