ALL SOLID-STATE LASER FOR BIOMEDICAL INSTRUMENTATION

大量的生物医学分析技术，如流式细胞仪和 共聚焦显微镜，利用激光诱导荧光检测。这 为生物医学研究提供高灵敏度的分析工具 临床诊断。在这些应用中最常用的激光器 是488 nm的Ar离子激光器，因为它与激发很好地匹配 使用最广泛的荧光素染料的波长。然而， 这种激光器不太适合这些应用：它相当大 而且效率很低，所以它会将大量多余的热量散失在 仪器和消耗相当大的电力。它也有有限的 使用寿命长，所以必须定期维修。 这一阶段第一阶段的目的是调查制定一项契约的可行性， 高效、坚固、长寿命的激光光源， 在生物医学中经济高效地替代Ar离子激光器 仪器。它将基于新的InGaAs频率倍增在一个 非线性晶体可实现约490 nm的波长，最佳 适用于这些应用程序。我们建议使用谐振倍频腔来 实现了良好的转换效率。第二阶段的后续行动将需要 开发一种用于生物医学仪器的490 nm光源。

Numerous biomedical analysis techniques, such as flow cytometry and confocal microscopy, utilize laser-induced-fluorescence detection. This provides a high sensitivity analysis tool for biomedical research and clinical diagnosis. The most commonly used laser for these applications is the 488nm Ar-ion laser, since it is well matched to the excitation wavelength of fluorescein dyes which are the most widely used. However this laser is less than optimal for these applications: it is fairly large and has low efficiency, so it dissipates much excess heat in the instrument and consumes substantial electrical power. It also has limited lifetime, so regular repair is necessary. This Phase I aims to investigate the feasibility of developing a compact, efficient, robust, long-lifetime laser source that would be a superior, cost-effective replacement for Ar-ion lasers in biomedical instrumentation.It would be based on new InGaAs frequency-doubled in a nonlinear crystal to achieve a wavelength of approximately 490nm, optimal for these applications. We propose to use a resonant doubling cavity to achieve good conversion efficiency. The Phase II follow-on would entail developing a 490nm source optimized for use in biomedical instruments.