NEW FLOW CYTOMETRY TECHNOLOGIES FOR CLINICAL HEMATOLOGY

自动化血细胞分析是临床上的一项常规功能 血液学实验室。我们都曾为此目的采集过样本， 但很少有人意识到，流式细胞仪 这些测试可能对RED的测量有基本的限制 血细胞体积，红细胞血红蛋白浓度，准确 白细胞亚群的分类。“高端”乐器 已经变得非常复杂和昂贵，以便“确保” 可以报告准确可靠的结果。在这方面仍有差距 提供一个低成本、易用的系统，可以提供准确的 和可靠的小区数据。 本提案描述了两种可应用于临床流程的新技术。 用于血液学分析的细胞术。通常与以下内容相关的问题 血红蛋白浓度测量和细胞体积干扰 红细胞测定及重复性问题 白细胞中的光散射和体积干扰 可以避免差动测量。这是由一种新的生物医学完成的 通过相移测量确定细胞干质量的仪器 利用双频HeNe激光和细胞体积染料的原理 排除。 终极仪器将以低成本提供独特的能力 一种准确制作红细胞、白细胞干质量和体积的装置 单细胞分析中的测量。

Automated blood cell analysis is a routine function in the clinical hematology laboratory. We have all had samples taken for such a purpose, but very few realize that the flow cytometric instrumentation performing these tests may have fundamental limitations regarding measurement of red blood cell volume, red blood cell hemoglobin concentration, and accurate classification of white blood cell subpopulations. 'High-end' instruments have become very complex and expensive in order to 'ensure' that an accurate and reliable result can be reported. There remains a gap in the availability of a low cost, easy to use system which can provide accurate and reliable cell data. This proposal describes two novel techniques applicable in clinical flow cytometry for hematologic analysis. Problems commonly associated with hemoglobin concentration measurements and cell volume interferences when making red blood cell measurements and problems of reproducibility of light scattering and volume interferences in the white blood cell differential measurement can be avoided. This is done by a new biomedical instrument that determines cell dry mass by measurements of phase shift using a two-frequency HeNe laser and cell volume by the principle of dye exclusion. The ultimate instrument will provide a unique capability as a low cost device for making accurate red and white blood cell dry mass and volume measurements in single cell analysis.