PREPARATIVE CHROMATOGRAPHY OF PROTEINS

高效液相色谱法（HPLC）已被证明是一种优秀的 纯化和定量医学上重要的蛋白质和肽的方法 例如变体和糖基化血红蛋白、乳酸脱氢酶 干扰素和胰岛素。 不幸的是，现有的HPLC柱 分析性质，不能重复纯化克或 公斤的合成或粗蛋白质的质量要求， 可能用于人类。 本申请描述了式I化合物的合成。 一系列专门设计的键合相液相色谱载体 用于蛋白质和肽的大规模制备纯化。 这样的支撑物将具有高容量和高分辨率，但也是可接受的。 刚性和稳定。 第二阶段的具体目标是综合、评价和选择 用于制备疏水和离子交换的制备载体 色谱法，开发方法来扩大合成程序， 50公斤的水平，并制定操作参数， 加载和分辨率，并最大限度地降低大型柱分析过程中的成本 这些支持。 因为这样的支持是不可用的，在目前的时间和非持久性 可压缩凝胶被用作替代品，其需求是毋庸置疑的。 生物技术和制药公司，他们正在纯化蛋白质， 肽将是主要的商业用户。

High performance liquid chromatography (HPLC) has proven to be an excellent method to purify and quantitate medically important proteins and peptides such as variant and glycosylated hemoglobins, lactate dehydrogenase, interferon and insulin. Unfortunately, existing HPLC columns are analytical in nature and are incapable of preparatively purifying grams or kilograms of synthetic or crude proteins to a quality demanded by their potential use in humans. This application describes the synthesis of a series of bonded phase liquid chromatography supports specifically designed for the large-scale preparative purification of proteins and peptides. Such supports would have high capacity and high resolution, yet also be rigid and stable. The specific aims of Phase II would be to synthesize, evaluate and select preparative supports for preparative hydrophobic and ion-exchange chromatography, to develop methods to scale the synthetic procedures to 50kg levels, and to develop operational parameters which would maximize loading and resolution and minimize cost during analyses on large columns of these supports. Because such supports are unavailable at the present time and nondurable compressible gels are used as substitutes, the need is unquestionable. Biotechnological and pharmaceutical firms who are purifying proteins and peptides would be the major commercial users.