MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

我们正在申请资金购买急需的 仪器，透射电子显微镜， 无失真成像和高加速能力 高对比度高倍率透射电压 电镜 利用免疫电子显微镜， 透射电子显微镜辅助心脏形态测量， 肺和分离的细胞，我们希望获得新的和有用的， 肺中免疫生物学反应的信息， 对氧毒性的反应，C5a诱导的炎症反应， 高血压性心脏病 利用免疫电子 我们计划用显微镜在分子水平上研究 C5转化酶在肺和膜攻击 配合物C5 - 9。 此外，我们还将研究 溶细胞性T细胞的特征及其与细胞因子的相互作用 靶向肿瘤细胞，因为它涉及它们溶细胞颗粒 内容 这些项目是NIH资助的赠款。 履行这些 研究项目将大大加强提供的 TEM要求。 我们也希望获得新的信息 关于C5-C5转化酶相互作用的化学计量 和C5-C9络合物的形成。 我们所有研究的最终目标是获得数据， 有用的信息，最终可以导致发展 预防和治疗模式。

We are applying for the funds to purchase a much needed instrument, a Transmission Electron Microscope with the capabilities for distortion free imaging and high accelerating voltage for high contrast high magnification transmission electron microscopy. Utilizing immunoelectron microscopy and transmission electron microscopy aided morphometry of hearts, lungs and isolated cells we hope to gain new and useful information in the immunobiological reactions in the lung in response to oxygen toxicity, C5a induced inflammation and in hypertensive heart diseases. By utilizing immunoelectron microscopy we plan to study at molecular levels the assemblies of C5 convertase in the lung and the membrane attack complexes C5-9. Additionally, we will study the structural features of the cytolytic T cells and their interactions with the target tumor cells, as it pertains to their cytolytic granule content. These projects are NIH funded grants. Fulfillment of these research projects will be greatly enhanced by availability of the TEM requested. We also hope to obtain new information regarding the stoichiometry of C5-C5 convertase interactions and of the C5-C9 complex formation. Ultimate goal of all our research is to obtain data to provide useful information that can ultimately lead to development of preventive and therapeutic modes.