CHEMOSTERILANT ACTIVITY OFJUVENILE HORMONE ANALOG--DUGESIA OR AEDES SECRETIONS

在拟议的生物医学研究中，首席研究员和 学生将试图中断的传播，发展和 亚洲虎蚊媒介伊蚊种群增长过程 Albopictus（Skuse）在美洲。 具体而言，长期或 最终目标是防止几种可能的传播， 地方性虫媒病毒，包括登革热和长曲棍球脑炎病毒， 美洲白纹伊蚊通过释放性 不育雄性和用杀幼虫剂处理繁殖生境 粘液分泌物中的物质。 这些方法应该能够 大大减少了Ae的数量。白纹伊蚊，因为没有 用于预防或治疗这些人类感染的治疗措施。 1980年，登革热的首次本土传播发生在 美国在35年后，随后在1986年。 为达致上述目标，建议的研究分五个阶段进行 (1)成年人口服治疗（实验室和田间笼舍研究），（2） （3）处理后F_1代的寿命 雄性，（4）雄性不育的持久性和（5）粘液分泌物 （提取、测试和鉴定粘液中的有毒物质）。 将通过口服治疗进行化学不育剂效果试验 成年阶段，阿。白纹伊蚊有三类幼虫， 苄基苯酚（J2419，J2644，J2706），苯并间二氧杂环戊烯（J2581，J2693）和 氨基甲酸酯（苯氧威）。 鸡蛋的非孵化率将被用作 用于计算在卵中诱导的不育性的标准。 杀幼虫剂 粘液分泌物的影响将通过一系列 不同龄期幼虫的实验室耐受性试验， 分泌物的浓度更高 另外， 将分析分泌物以分离并确定（通过使用 HPLC）测定粘液中的目标杀幼虫物质。

In the proposed biomedical research, the principal investigator and students will attempt to interrupt the dispersal, development and population growth process of the Asian tiger mosquito vector, Aedes albopictus (Skuse) in the Americas. Specifically, the long term or ultimate objective is to prevent the possible transmission of several endemic arboviruses including Dengue and LaCrosse encephalitis virus in the Americas by Aedes albopictus, through the release of sexually sterilized males and the treatment of breeding habitats with larvicidal materials from mucous secretions. These methods should be capable of drastically reducing populations of Ae. albopictus, since there is no therapeutic measure for preventing or treating these human infections. In 1980, the first indigenous transmission of dengue occurred in the United States in thirty-five years and subsequently in 1986. To obtain the objectives the proposed study is divided into five phases (1) oral treatment of adults (laboratory and field cage studies), (2) effects of juveniods on the F1 generation, (3) longevity of the treated males, (4) permanency of male sterility and (5) mucous secretions (extract, test and identify toxic substances in the mucous). Tests for chemosterilant effect will be conducted through oral treatment of the adult stage, Ae. albopictus with three classes of juveniods, benzylphenols (J2419, J2644, J2706), benzodioxoles (J2581, J2693) and a Carbamate (fenoxycarb). Non-hatchability of eggs will be used as a criterion for calculating sterility induced in eggs. The larvicidal effect of mucous secretions will be determined through a series of laboratory tolerance tests with the different larval instars at two or more concentration levels of the secretions. Also, samples of the mucous secretions will be analyzed to separate and determine (through use of HPLC) the target larvicidal material in the mucous.