TURBELLARIA AND PLANARIANS AS BIO-CONTROL FOR CULICIDAE

拟议的研究旨在评估和调查 替代杀虫剂以抑制 不成熟阶段的蚊，感染人类， 导致衰弱性疾病如脑炎的微生物， 疟疾、登革热、丝虫病和黄热病。 的疗效 不同种类的淡水真涡虫（三枝虫： Turbellaria）将作为替代生物防治剂进行研究。 标本将在密西西比和路易斯安那州采集。 的 本研究分为五个阶段：（1）实验室培养 以及大量生产真涡虫， 通过光周期、营养、切片和 其他因素（2）捕食者-猎物之间的相互作用五个或更多 成熟的真涡虫种类以及实验室和野外种群 （3）对杀幼虫剂的调查 从真涡虫B离心收获的粘液的潜力 和食物刺激（4）不同物种的耐受程度 涡虫对不同剂量昆虫生长调节剂的反应 （5）人工林的田间研究 对比实验室结果。 这项研究将试图回答这些问题，并作为一个 刺激进一步调查，旨在消除 蚊子传播的疾病，持续作为主要的， 世界各地的疾病和死亡。 比如说， 圣路易恩塞格林持续爆发 在过去的十年里，这个国家的南部， 1975年，30个州报告了2000多起病例。 如果 粘液分泌物被确定为有效的杀幼虫剂， 事实上，这将是公共卫生的一个重大突破， 生物医学的重要性 长远目标和基本原则 这项研究应该激励生物医学导向的学生， 扩大机构研究。

The proposed research seeks to evaluate and investigate an alternative to pesticides for population suppression of the immature stages of Culicides (mosquitoes) that infect man with organisms that cause debilitating diseases such as encephalities, malaria, dengue, filariasis and yellow fever. The efficacy of different species of the fresh-water planarians (Tricladida: Turbellaria) will be investigated as alternative bio-control agents. Specimen will be collected in Mississippi and Louisiana. The proposed study is divided into five phases: (1) laboratory culturing and mass production of planarians by accelerating the process of a sexual reproduction through photoperiod, nutrition, sectioning and other factors (2) predator-prey interaction between five or more species of mature planarians and laboratory and filed population of mosquito vectors (3) an investigation into the larvicidal potential of mucous harvested from planarians b centrifugation and food stimulation (4) degree of tolerance by various species of planarians to different dosage levels of insect growth regulations under laboratory and field conditions (5) field studies in artificial ponds to compare laboratory results. The study will attempt to answer these questions and act as a stimulus for further investigation, aimed at the elimination of mosquito-borne disease which persist as the major, cause of illness and deaths in various parts of the world. For example, there has been a continued outbreak of St. Louis Encephalitis in the Southern portion of this country over the past decade, with more than 2000 cases reported from 30 states in 1975. If the mucous secretion is determined to be a potent larvicide, this indeed, would be a significant breakthrough of public health or biomedical importance. The long-range goal and basic principle of this study should inspire biomedical oriented students and broaden institutional research.