CANCER, LIPID AND STRESS RESEARCH

拟议的计划旨在激励少数族裔教职员工。 会员从事生物医学研究，加强 院校培养研究生和健康学生的能力 专业学习，为少数民族学生提供机会 获得生物医学研究经验，并对学生感兴趣 追求生物医学科学领域的研究事业。为此， 该计划包括的子项目包括： 1)脂解酶及其底物 胰腺、小肠和肾脏。比较级的 小鼠胰腺内源性脂质变化的研究 豚鼠和大鼠的肠、肾通过 检测它们各自的脂解能力 经体外孵育的产物。脂类成分分析 氯仿：对照样品和培养样品的甲醇提取物 通过多种形式的薄层层析将提供 数据的基础。将各自的TLC观察结果关联起来 伴随发情周期相关的纤溶酶原改变，肾脏 标本将用于纤溶酶原组织化学。 2)与压力相关的检测和控制 生理变量。四个变量的考察 (外周温度、脉搏通过时间、心率和皮肤 电导性)决定人类的辨别能力 并自愿控制与之相关的某些生理功能 压力引起的或心身障碍。检测和检测 控制是生物反馈使用的行为方法的基础 训练、整体治疗、冥想技巧和压力 管理层。 铂-胸腺嘧啶联合化疗 癌细胞的数量。超微结构和组织化学研究 铂-胸腺嘧啶与博莱霉素联合应用的研究进展 使用腹水肉瘤-180细胞在体外和体内。剂量 将监测使用联合疗法限制副作用 瑞士人肠肾癌和正常细胞的研究 白化小鼠。联合用药的作用方式为 通过观察它们对碱性的影响进行组织化学测定 磷酸酶和钙激活的ATPase。

The proposed program is designed to motivate minority faculty members to engage in biomedical research, strengthen the institution's capabilities to train students for graduate and health professional study, provide opportunities for minority students to gain biomedical research experience, and interest students in pursing research careers in the biomedical sciences. To this end, the subprojects included in the program are: 1) LIPOLYTIC ENZYMES AND THEIR SUBSTRATES OF PANCREAS, SMALL INTESTINE, AND KIDNEY. A comparative study of the variety of endogenous lipids in the pancreas, small intestine, and kidney of the guinea pig and rat through examination of their respective lipolytic capacities as expressed by the products of in vitro incubation. Lipid analysis of chloroform:methanol extracts of control and incubated samples via multiple forms of thin layer chromatography will provide the basis of the data. To correlate the respective TLC observations with estrous cycle related plasmalogen changes, kidney specimens will be used for the plasmalogen histochemistry. 2) DETECTION VERSUS CONTROL OF STRESS RELATED PHYSIOLOGICAL VARIABLES. Investigation of four variables (peripheral temperature, pulse transit time, heart rate, and skin conductance) determining the ability of humans to discriminate and voluntarily control certain physiological functions related to stress-induced or psychosomatic disorders. Both detection and control underlie the behavioral approach used in biofeedback training, holistic healing, meditation techniques, and stress management. 3) PLATINUM-THYMINE IN COMBINATION CHEMOTHERAPY OF CANCER CELLS. Ultrastructural and histochemical studies of the combined application of platinum-thymine and bleomycin using ascites sarcoma-180 cells in vitro and in vivo. Dose limiting side effects using combination therapy will be monitored on cancer and normal cells of the intestine and kidney in Swiss Albino mice. The mode of action of the combined drugs will be determined histochemically by observing their effect on alkaline phosphatase and calcium-activated ATPase.